Articles

Quality Assessment of Orthosiphon stamineus Benth by using High Performance Liquid Chromatography Fingerprinting

Author

KHAMSAH SURYATI MOHD, GABRIEL AKOWAH & ZHARI ISMAIL

Proceeding

The 21st Annual Seminar of the Malaysian Natural Product Society.(Herbal Medicine:Natural Products S ciety), Palm Garden, Putrajaya Malaysia.

Date

22/11/2005

Keyword

Fingerprinting, Orthosiphon stamineus Benth, standardization

Abstract

The trade in and use of crude indigenous herbal products is largely unregulated in many parts of the world. Orthosiphon stamineus Benth is a popular medicinal plant in Southeast Asia. In Indonesia and Malaysia, the decoction of the leaves is used to alleviate bladder and kidney discomfort, treat arteriosclerosis, gout and rheumatism. In order to assess the starting material from different growers and suppliers, we used the HPLC profiles to identify characteristic of chemical fingerprinting of this plant via comparison of retention data. Four standard compounds, namely sinensetin (SN), eupatorin (EU), 3’-hydroxy-5, 6,7,4’-tetramethoxyflavone (TMF) and rosmarinic acid (RA) were used. By using the multiple markers, the proportional of the concentration of the markers can be determined. The proportion of markers concentrations decreased in the order of RA> EU> SN> TMF. These proportional patterns of markers are constant and can be seen in all samples. These results show that HPLC fingerprinting can provide aid model in the assessment of quality control of raw herbal material.