Articles

Antibacterial, antisecretory and antihemorrhagic activity of Azadirachta indica used to treat cholera and diarrhea in India

Author

Thakurta P, Bhowmik P, Mukherjee S, Hajra T. K, Patra A, Bag P. K.

Journal

Journal Ethnopharmacology

Volume

111

Issue ID

3

Page

607-12

Date

2007 May 22.

Keyword

Alternative medicine
Animals
Anti-Bacterial Agents/administration & dosage
Anti-Bacterial Agents/pharmacology*
Azadirachta*
Cholera/drug therapy*
Diarrhea/drug therapy
Dose-Response Relationship, Drug
Drug Resistance, Multiple, Bacterial
Gastrointestinal Hemorrhage/drug therapy
India
Intestinal Secretions/drug effects
Medicine, Ayurvedic
Mice
Mice, Inbred BALB C
Microbial Sensitivity Tests
Phytotherapy
Plant Extracts/administration & dosage
Plant Extracts/pharmacology*
Plant Leaves
Plants, Medicinal
Vibrio cholerae/drug effects*

Abstract

Indigenous uses of Azadirachta indica A. juss (Maliaceae) (locally known as neem) leaves in different parts of India for curing gastrointestinal disorder such as diarrhea and cholera is wide spread. The objective of the present study was to evaluate the antibacterial and antisecretory activity of neem extract against Vibrio cholerae, a causative agent of watery diarrhea such as cholera. The methanol extract of neem leaf was tested for its antibacterial, antisecretory and antihemorrhagic activity against Vibrio cholerae. Azadirachta indica extract had  significant antibacterial activity against the multi-drug-resistant Vibrio cholerae of serotypes O1, O139 and non-O1, non-O139. The minimum inhibitory concentration reached by 50% (MIC50) and 90% (MIC90), and minimum bactericidal concentration for the extract were 2.5, > 5, and 10 mg/ml, respectively. Neem extract showed antisecretory activity on Vibrio cholerae induced fluid secretion  in mouse intestine with inhibition values of 27.7%, 41.1%, 43.3%, 57.0%, and 77.9% at doses of 100, 200, 300, 450 and 1800 mg/kg, respectively. Oral administration of the extract inhibited hemorrhage induced by Vibrio cholerae in  mouse intestine at a dose > or = 300 mg/kg. The results obtained in this study give some scientific support to the uses of neem employed by the indigenous people in India employed for the treatment of diarrhea and dreadful disease cholera.

Language

English