Morinda citrifolia L. (Fruits)

MALAYSIAN HERBAL MONOGRAPH

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Mengkudu Fruit

 

Morinda citrifolia L.

Rubiaceae

mengkudu1 mengkudu2 mengkudu3
(b) (c)
mengkudu4 mengkudu5
(a) (d) (e)
Figure 1 : M. citrifolia. (a) Whole plant; (b)-(c) fruits; (d) leaves; (e) flowers. (Photos courtesy of Thiyagu, MARDI, 2012)

DEFINITION

Mengkudu fruit consists of dried partially ripe fruits of M. citrifolia L. (Rubiaceae) [1].

SYNONYM

Morinda bracteata Roxb. [2]

VERNACULAR NAMES

Mengkudu, mengkudu besar (Malay); hai ba ji, wu ning luo ling kuo (Chinese); nunaakai, nunavu(Tamil); Indian mulberry (English). [2,3,4,5]

CHARACTER

The fruit is yellow to green in colour with bitter taste. The ripe fruit has a strong butyric acid-like rancid smell. [6]

IDENTIFICATION

Plant Morphology

M. citrifolia grows in shady forests and on open rocky or sandy shores; it is a shrub or small-medium sized tree (3-10 m) with quadrangular or somewhat rounded branches and evergreen. The leaves are large, simple, alternate, broadly elliptic to oblong shape (10-30 x 5-15 cm), dark green, glossy, wavy and prominently-veined. The 5-lobed white tubular-like fragrant flower is small (1.25 cm long) and borne in a globose head (2.5 cm across). The heads develop into compound fruits composed of many small drupes; the fruit is ovoid, ellipsoid or roundish (3-10 x 3-6 cm) with an embossed appearance, slightly wrinkly, waxy, semi-translucent skin, and turns from green to yellow and to almost white as it ripens; the fruit surface is faintly patterned with 4- to 6-sided outlines, each with a central "eye"; the pulp is fleshy and juicy, dull-yellow or yellowish-white and gelatinous when the fruit is ripe; it has numerous hard oblong-triangular reddish-brown pits, each containing 4 seeds about 3.5 mm long. [6,7]

Microscopy

The fruit powder contains thin-walled parenchyma cells, exocarp and sparsely distributed thin strands of vascular tissue containing tracheary elements with annular, spiral and rarely, pitted secondary walls; acicular calcium oxalate crystals, starch granules and oil globules [1,8].

micro1 micro2
(a) (b)
micro3 micro4
(c) (d)
Figure 2 : Microscopic characters of M. citrifolia fruit powder. (a) Thin-walled parenchyma cells (magnification 400X); (b) a bundle of spiral vessels (magnification 400X); (c) acicular calcium oxalate crystals (magnification 100X); (d) exocarp (magnification 100X).

Colour Tests

Observed colour of solution after treatment with various reagents:

H2SO4 (conc.) :

Brown to dark brown

5% NaOH :

Light brown to brown

Thin Layer Chromatography (TLC)

 
Test Solution : Weigh about 1.0 g of M. citrifolia dried fruit powder in a 50 mL screw-capped conical flask and add 10 mL of ethanol into the flask. Shake the mixture for 15 min. Filter the mixture and evaporate the filtrate on a water bath to dryness. Reconstitute the residue with 0.5 mL of methanol.
Standard Solution : Dissolve 2.0 mg of scopoletin standard in 20 mL of methanol to give 100 µg/mL solution.
Stationary Phase : HPTLC Silica gel 60 F254, 5 x 10 cm.
Mobile Phase :

n-Butanol-acetic acid-water, 5:1:4 (v/v)

Preparation of butanol-acetic acid-water, 5:1:4  (v/v)

Saturate butanol with water at the ratio of 1:1 (v/v), in a separation funnel for 12 hr. Collect the upper organic layer and saturate it with acetic acid and water at a ratio of 5:1:4 (v/v) in a separation funnel for 12 hr. Collect the upper organic layer and use as the mobile phase.

Application :

(a) Scopoletin standard solution (S); 20 µL, as a band

(b) Ethanol extract of M. citrifolia fruit (L); 8 µL, as a band

Development Distance : 8 cm
Drying : Air drying
Detection : (a) UV 254 nm; (b) UV 366 nm

 

morinda tlc
Figure 3 : TLC profiles of scopoletin (S) and ethanol extracts of M. citrifolia fruits  (L) observed under (a) UV at 254 nm and (b) UV at 366 nm.

High Performance Liquid Chromatography (HPLC)

 

Test Solution : Extract about 1.0 g of M. citrifolia dried fruit powder in a 50 mL screw-capped conical flask and add 4 mL of methanol. Sonicate in a water bath of 50°C for 60 min. Cool the mixture and allow the insoluble matter to settle. Filter 3 mL of the upper solution through a 0.45 µm syringe filter and inject the filtrate into the HPLC column.
Standard Solution : Dissolve 2.0 mg of scopoletin standard with 50 mL of methanol to produce 40 µg/mL solution.
Chromatographic System :
Detector: UV 350 nm
Column: C18  (5 µm, 4.6 mm I.D x 250 mm)
Column oven temperature: 30°C
Flow rate: 1.2 mL/min
Injection volume: 10 µL

Mobile Phase (Gradient mode) :

 table

System suitability requirements : Perform at least five replicate injections of the scopoletin standard solution (40 µg/mL). The requirements of the system suitability parameters are as follow:
  1. Symmetry factor (As) is not more than 1.5.
  2. Percentage of relative standard deviation (RSD) of the retention time (tr) for scopoletin is not more than 2.0%.
Acceptance criteria :
  1. Retention time (tr) of scopoletin in the test solution is similar to the tr of scopoletin in the standard solution.
  2. The resolution (Rs) value between peak of scopoletin and peak A in the test solution should not be less than 1.5.
  3. The Ultraviolet (UV) spectrum of scopoletin in the test solution is similar to the UV spectrum of scopoletin in the standard solution (optional supportive data). 

 

hplc1
Figure 4 : HPLC chromatogram of scopoletin standard solution (40 µg/mL) at tr = 15.178 min

 

hplc2
Figure 5 : HPLC chromatogram of methanol extract of M. citrifolia fruit showing peaks corresponding to scopoletin (tr = 15.187 min) and peak A (tr = 16.145 min)

 

mengkudu jpg
Figure 6 : UV spectrum of scopoletin standard solution (40 µg/mL) and methanol extract of M. citrifolia fruit 

PURITY TESTS

Foreign Matter
Not more than 2%

 

Ash Contents
Total ash : Not more than 6%
Acid-insoluble ash : Not more than 1% 

 

Loss on Drying
Not more than 9%

 

Extractive Values
Water-soluble extracts
Hot Method        : Not less than 39%
Cold Method : Not less than 30%
Ethanol-soluble extracts
Hot Method : Not less than 17%
Cold Method : Not less than 10%

SAFETY TEST

Heavy Metals
Arsenic                      Not more than 5.0 mg/kg
Mercury : Not more than 0.5 mg/kg
Lead : Not more than 10.0 mg/kg
Cadmium : Not more than 0.3 mg/kg

 

Microbial Limits
Total aerobic microbial count                         
 :   Not more than 105 cfu/g                               
Total yeast and mould count : Not more than 104 cfu/g
Bile-tolerant gram negative bacteria : Not more than 104 cfu/g

 

Specific Pathogens 
Salmonella  spp.                          
:   Absent in 25 g                                    
Escherichia coli : Absent in 1 g
Staphylococcus aureus : Absent in 1 g
Pseudomonas aeruginosa : Absent in 1 g

CHEMICAL CONSTITUENTS

The fruits juice has been found to contain glycosides (e.g. 1-O-(3’-methylbut-3’-enyl)-β-D-glucopyranose, 1-n-butyl-4-(5’-formyl-2’-furanyl)methyl succinate, 4-epiborreriagenin, 1-n-butyl-4-methyl-2-hydroxysuccinate, 1-n-butyl-4-methyl-3-hydroxysuccinate, α-glucopyranose, β-glucopyranose, deacetylasperulosidic acid, asperulosidic acid, scopoletin). [9]

Butanol extract of the fruits has fatty acid glucosides (e.g. 1,6-di-O-octanoyl-β-D-glucopyranose, 6-O-(β-D-glucopyranosyl)-1-O-decanoyl-β-D-glucopyranose, 6-O-(β-D-glucopyranosyl)-1-O-octanoyl-β-D-glucopyranose, 6-O-(β-D-glucopyranosyl)-1-O-hexanoyl-β-D-glucopyranose, 2,6-di-O-(β-D-glucopyranosyl)-1-O-octanoyl-β-D-glucopyranose) and others (e.g. rutin, asperulosidic acid). [10,11]

Ethanol extract of the fruits contains polysaccharides (e.g. pectin polysaccharides, type II arabinogalactan, xyloglucan, heteroxylan, heteromannan) and vitamins (e.g. ascorbic acid, provitamin A). [12]

Methanol extract of the fruits has anthraquinones (e.g.1,6-dihydroxy-5-methoxy-2-methoxyanthraquinone, 1,5,7-trihydroxy-6-methoxy-2-methoxymethylanthraquinone, 1,5,15-tri-O-methylmorindol, 1,5-dimethylmorindol, 1,3-dimethoxyanthraquinone, alizarin, anthraquinone), flavonoids (e.g. quercetin, kaempferol), lignans (e.g. (+)-3,4,3’4’-tetrahydro-9,7’α-epoxylignan-7α,9’-lactone), iridoids (e.g. 6a-hydroxyadoxoside, 6b,7b-epoxy-8-epi-splendoside, morinaphthalenone, borreriagenin, citrifolinin B epimer a and b, cytidine, epi-dihydrocornin, scopoletin, isoscopoletin, 3,3’-bisdemethyltanegool, (-)-pinoresinol, (-)-3,3’-bisdemethylpinoresinol, americanin A, narcissoside), alkaloids (e.g. proxeronine, xeronine), glucosides (e.g. D-glucose, D-mannitol methyl a-D-fructofuranoside, methyl b-D-fructofuranoside, nicotifloroside, b-sitosterol 3-O-b-D-glucopyranoside, 6-O-(β-D-glucopyranosyl)-1-O-octanoyl-β-D-glucopyranose, 6-O-(β-D-glucopyranosyl)-1-O-hexanoyl-β-D-glucopyranose, 3-methyl-3-butenyl 6-O-β-D-glucopyranosyl-β-D-glucopyranoside, 2-O-(β-D-glucopyranosyl)-1-O-octanoyl-β-D-glucopyranose, 2-O-(β-D-glucopyranosyl)-1-O-hexanoyl-β-D-glucopyranose, noniosides E-H, 2,6-di-O-(β-D-glucopyranosyl)-1-O-octanoyl-β-D-glucopyranose, 6-O-(β-D-glucopyranosyl)-1-O-octanoyl-β-D-glucopyranose) and others (e.g. vanillin, asperuloside, asperulosidic acid, deacetylasperuloside, dehydromethoxygaertneroside). [13,14,15,16,17,18,19,20,21,22].

Dichloromethane extract of the fruits has been found to contain derivatives of acids (e.g. hexanoic acid, octanoic acid, decanoic acid, nonanoic acid, heptanoic acid, butanoic acid, acetic acid, butanoic acid, 2-methylbutanoic acid, 2-methylpropanoic acid, 3-methylthiopropanoic acid, benzoic acid, hexanedioic acid, undecanoic acid, lauric acid, myristic acid, palmitic acid, linoleic acid, oleic acid (Z,Z,Z)-8,11,14-eicosatrienoic acid), alcohols (e.g. 1-butanol, 3-methyl-3-buten-1-ol, 3-methyl-2-buten-1-ol,1-hexanol, benzyl alcohol, eugenol, (Z,Z)-2,5-undecadien-1-ol) and others (e.g. methyl hexanoate, ethyl hexanoate, methyl ocatanoate, methyl 5-nonenoate, methyl decanoate, methyl 3-methylthio-propanoate, ethyl octanoate, ethyl decanoate, methyl palmitate, methyl elaidate, methyl oleate, 3-hydroxy-2-butanone, 2-heptanone, (E)-6-dodeceno-γ-lactone, (Z)-6-dodeceno-γ-lactone, hexamide, limonene, scopoletin, vomifoliol). [23]

Hexane extract of the fruits contains sulfur compounds (e.g. methanethiol, S-methyl thioacetate, dimethyl disulfide, methyl 3-methylthiopropanoate), terpenes (e.g. linalool oxide, (Z)-3,7-dimethyl-1,3,6-octatriene, (+)-4-carene, D-limonene, ocimenol, terpineol), derivatives of acids (e.g. butanoic acid, decanoic acid, 2E,4Z,7Z-decatrienoic acid, hexanoic acid, octanoic acid, 2-octenoic acid, nonanoic acid, heptanoic acid, acetic acid, formic acid) and others (e.g. 1-butanol, 1-methyl-3-buten-1-ol, 1-methyl-2-buten-1-ol, 2-methyl-2-buten-1-ol, 3-methyl-3-buten-1-ol, 3-methyl-2-buten-1-ol, 3-methyl-2-butanone, benzyl alcohol, acetaldehyde, 2-methylbutanal, 3-methylbutanal, 2-pentanone, 2-hexanone, hexanal, 2-heptanone, 2-hexenal, furfural, benzaldehyde, ethanol, benzyl alcohol, ethyl acetate, butyl acetate, methyl 2-methylpropanoate, methyl butanoate, ethyl butanoate, butyl butanoate, 4-pentenyl butanoate, methyl 2-methylbutanoate, methyl 3-methylbutanoate, 3-methyl-3-buten-1-yl-3-methylbutanoate, methyl hexanoate, ethyl hexanoate, butyl hexanoate, 4-pentenyl hexanoate, 3-methyl-3-buten-1-yl hexanoate, hexyl isovalerate, methyl heptanoate, methyl ocatanoate, ethyl octanoate, butyl octanoate, 3-methyl-3-buten-1-yl octanoate, methyl 2-octenoate, methyl 3-octenoate, methyl 6-octenoate, methyl nonanate, methyl 5-nonenoate, methyl decanoate, ethyl decanoate, methyl 4-decenoate, ethyl 4-decenoate, methyl salicylate, methyl hexadecanoate). [24,25]

MEDICINAL USES

Uses described in folk medicine, not supported by experimental or clinical data

The ripe fruit of M. citrifolia is traditionally used for relief of sore throat, boil, carbuncle, stomach ulcer and inflamed elbow. The fruit juice is consumed to prevent adverse effects of kava (Piper methysticum). The fruit pounded with sugar cane and kava root is used for tuberculosis. The charred unripe fruits have been applied with salt for gum disorders, while the fresh young fruit mashed with salt is applied on deep cuts and poulticed over broken bones. [3,6,26]

Biological and pharmacological activities supported by experimental data

Antioxidant activity
Ethyl acetate extract of M. citrifolia fruit (4 mg) had strong antioxidant activity comparable to tocopherol and butylated hydroxyl toluene [26].
 
Antimicrobial activity
Methanol extract of M. citrifolia fruit (100 mg/mL) showed strong activity against gram-negative bacteria (Salmonella paratyphi A) and moderate activity against gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, Lactobacillus lactis, Streptococcus thermophilus, Pseudomonas aeruginosa) and other gram-negative bacteria (Salmonella typhi, Escherichia coli, Vibrio harveyi, Klebsiella pneumonia, Shigella flexneri, Salmonella paratyphi A, Aeromonas hydrophilam, Vibrio cholera, Chromobacterium violaceum and Enterobacter faecalis). The ethyl acetate extract showed moderate activity against all aforementioned bacteria except for P. aeruginosa and K. pneumonia. [28]
 
Antifungal activity
Ripe fruit juice of M. citrifolia showed antifungal activity against Candida albicans with partial growth inhibition at a concentration of 40 mg/mL and complete growth inhibition at a concentration of 50 mg/mL after 30 min contact time [26]. The methanol and ethyl acetate extracts of M. citrifolia fruit showed strong activity against Trichophyton mentagrophytes with 79.3% and 62.06% inhibition, respectively. Methanol extract of the fruit also showed moderately strong activity (close to 50% inhibition) against Penicillium sp., Fusarium sp. and Rhizopus sp. The methanol, ethyl acetate and hexane extracts weakly inhibited Candida albicans and Aspergillus sp. [29]
 
Anti-angiogenic activity
Fruit juice of M. citrifolia (5% and 10%) was found to significantly inhibit (P<0.001) the initiation of new placental vessels and reduce the growth rate and proliferation of newly developing capillary sprouts compared to control explants using an in vitro angiogenesis assay. The 10% of M. citrifolia fruit juice was able to induce vessel degeneration and apoptosis of developed capillary vessels based on MTT and TUNEL assays, respectively. It was also effective in inhibiting capillary initiating of the human breast tumor explants. [30]
 
Cytotoxic activity

Methanol (1:6 dilution of crude extract) and ethyl acetate (1:3 dilution) extracts of M. citrifolia fruit inhibited almost 50% of the human laryngeal epithiloma (Hep2) cells [27]. The fruit extract also inhibited retinoblastoma Y79 cell lines (IC50 800 µg/mL) [31].

 
Antidyslipidemic activity

Aqueous-ethanolic (70%) extract of M. citrifolia fruit administered orally (1000 mg/kg) to tyloxapol-induced male Sprague Dawley rats caused significant reduction in total cholesterol level (P< 0.01) and triglyceride level (P< 0.05). The extract also significantly (P< 0.05) reduced total cholesterol, triglyceride, low density lipoprotein-cholesterol, atherogenic index and total cholesterol-high density lipoprotein-cholesterol ratio of high fat diet-induced dyslipidemic adult Sprague Dawley rat models [32].

 
Hypoglycemic activity

Fermented fruit juice of M. citrifolia given orally to male Sprague Dawley rats (2 mL/kg) twice daily for 20 days reduced fasting blood glucose by 52.6% [34].

 
Hepatoprotective activity
Fruit juice of M. citrifolia (20%) given to carbon tetrachloride (CCl4)-induced female Sprague Dawley rats for 7 days showed significant reduction in hepatotoxic lesions (P< 0.001), serum alanine amino transferase (P< 0.01) and aspartate aminotransferase (P< 0.05) levels. In a correlative time-dependent study, 10% of the juice displayed delayed appearance of damaged hepatocytes with a decreased number of necrotic foci (80%) and apoptosis (50%), suggesting that the juice protects the liver from acute CCl4 exposure [34].
 
Immunomodulatory effect
M. citrifolia fruit juice and juice concentrate (1 and 5 mg/mL) stimulated cannabinoid 2 (CB2) (54-224%) but inhibited cannabinoid 1 (CB1) (14-172%) receptors in vitro. The fruit juice administered orally (100 mL/day) to male mice for 16 days decreased the production of IL-4, but increased the production of IFN-γ cytokines. [35]

SAFETY INFORMATION

Preclinical  study(toxicology studies)

Acute toxicity
Oral single dose acute toxicity study of M. citrifolia dried fruit powder on female Sprague Dawley rats (aged between 8 and 12 weeks old) showed no toxic effects on the parameters observed, including behaviors, body weight, food and water intake. All rats were observed for 14 days prior to necropsy. No death was found throughout the study period. Necropsy revealed no significant abnormality. LD50 value was determined as > 2000 mg/kg. [36]

In acute oral toxicity studies with rats, the juice and puree of Tahitian M. citrifolia fruit had LD50 values higher than 15,000 mg/kg body weight, [37,38] while the fruit concentrate had LD50 value higher than 5000 mg/kg body weight [39].

The ethanol and aqueous extracts of M. citrifolia fruit were administered intraperitoneally at a single dose to 21-day old female mice. The LD50 of the ethanol and aqueous extracts were found to be 3500 mg/kg and 7500 mg/kg respectively. [40]

Sub-acute toxicity
Aqueous extract of M. citrifolia fruit (5.1% total solids) administered via oral gavage at a dose of 1000 mg/kg body weight to both male and female Sprague Dawley rats for 28 days showed no adverse effects on body weight, food consumption, haematological, clinical chemistry and histopathological parameters [41].
 
Sub-chronic toxicity
Fruit juice of Tahitian M. citrifolia fruit was administered by oral gavage at a daily dose of up to 80 mL/kg for 13-weeks to Sprague Dawley rats showed no adverse clinical signs, abnormal food consumption and weight gain, as well as no negative impact on the clinical chemistry, haematological and histopathological parameters. The No Observed Adverse Effect Level (NOAEL) was 80 mL/kg body weight/day. [42]

Others (Adverse reaction, contraindication, side effect, warning, precaution)

Adverse reaction
Reported adverse effects of the M. citrifolia fruit juice include allergic reactions, diarrhoea, nausea and skin rash [43].
 
Contraindication
Owing to possible teratogenicity, the aqueous extract and juice of M. citrifolia fruit should not be taken during pregnancy [44].
 
Warnings
M. citrifolia fruit juice include allergic reactions, diarrhoea, nausea and skin rash [43]. Consumers with liver disorders should seek advice from their health care professionals before taking any fruit juice preparations containing M. citrifolia. There were cases reported on hepatotoxicity after consuming noni juice but no convincing evidence for a causal relationship could be established which suggests that some individuals have a particular sensitivity for hepatotoxic effects to noni fruit products. [45]

DOSAGE

The recommended daily intake of M. citrifolia fruit juice is 30 mL/day (equivalent to around 0.5 mL/kg body weight for a 60 kg adult) [46].

STORAGE

Store below 30˚C. Protect from light and moisture.

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Latin name : Morinda bracteata Roxb., Morinda littoralis Blanco [1].