Phytochemical Studies Of Mesua Corneri (Linn.) And Garcinia Mangostana (Linn.) And Their Biological Activities

Author

SHEIKH AHMAD IZADDIN SHEIKH MOHD GHAZALI

Date

2006

Keyword

Garcinia mangostana L, Mesua corneri L., phytochemical studies, biological activity , antimicrobial assay, antifungal activity

Abstract

Roots of Garcinia mangostana L. and the stem bark of Mesua corneri L. were chemically investigated. Detail phytochemical studies on the roots of Garcinia mangostana L. and the stem bark of Mesua corneri L. have resulted in the isolation of eleven compounds. The structures of these compounds were elucidated using spectroscopic experiments namely NMR, IR, UV and MS. The root bark of Garcinia mangostana L. furnished six xanthones, α-mangostin, ß-mangostin, γ-mangostin, garcinone-D, mangostanol and gartanin. Up to now, research has only been carried out on the fruit hull and the stem bark of this plant. There have been no studies yet on the root bark of Garcinia mangostana L. Meanwhile, investigations on stem bark of Mesua corneri L gave three triterpenoid, stigmasterol, fridelin, friedelan-l,3-dione and two xanthones, rubraxanthone and Inophyllin B. So far, there has been no reports at all on this plant. The crude hexane and chloroform extracts of Mesua corneri L. stem bark were active against HL-60 cell line with IC50 values of less than 30 µg/ml. The crude hexane and chloroform extracts of Garcinia mangostana L. root bark were found to be active against CEM-SS cells line with IC50 values of less than 30 µg/ml. Meanwhile, the y-mangostin gave a significant activity with an IC50 value of 4.7 µg/ml. This is a new activity for this plant. The antimicrobial assay was carried out towards four pathogenic bacteria, MethicilJin Resistant Staphylococcus aures, Pseudomonas aeruginosa, Salmonella typhimurium and Bacillus subtilis. Most of the crude extracts tested against these microbes gave only moderate or weak activities. The larvicidal tests performed against the larvae of Aedes aegypti. The crude hexane and chloroform extracts of Garcinia mangostana L. showed a strong activity against the larvae with LC50 values of less than 100 µg/ml. The pure compounds α-mangostin and γ-mangostin gave good activities with LC50 value of 18.4 and 32.4 ug/ml respectively .The crude hexane and choloroform extracts of Mesua corneri L. showed a good activities against the larvae and gave LC50 values of less than 100 µg/ml. Rubraxanthone showed a strong activity against the larvae with a LC50 value of 18.4 µg/ml. These activities have not been reported before and this is a new finding. The antifungal activity testing of the plant extracts were carried out against the fungi Candida albican, Aspergillus ochraceaus, Sacchoromyces cerevisiae and Candida lypolytica. No activity was observed for all the crude extracts.