Insulinotropic Properties Of Several Malaysian Herbs And Ganoderma Species

Author

MOHD SAUFI BASTAMI

Date

2006

Keyword

Malaysian herbs, Ganoderma sp, Gynura procumbens, insulinotropic properties, anti-hyperglycemic , blood glucose level,

Abstract

Diabetes Mellitus incident is currently one of the major and common diseases in Malaysia and increasing every year. To date, no herbs or drugs are able to completely cure the disease. In this study, insulinotropic properties of Malaysian herbs were investigated using pancreatic p cells BRIN-BD11. Three out of 13 herbs samples studied were belongs to fungi (Ganoderma sp). The studied showed seven samples are able to induce insulin secretion over 200 % by BRIN-BD11 cell lines. Based on traditional practitioner reports, Gynura procumbens was choosed for further investigation on its anti-hyperglycemic properties. Glucose tolerance test were carried on normal and streptozotocin-induced diabetic rats showed at 1000 mg/kg/ b.w  Gynura procumbens methanolic extract was able to reduce blood glucose level in rats. Toxicity towards BRIN-BD11 cells was analyzed using MTT assay and alkaline comet assay. Toxicity levels at IC50 was 300 µg/ml using MTT assay. Furthermore, Alkaline Comet Assay has proved this toxicity is not due to DNA damage in BRIN-BD11 cells. Acute toxicity was carried on ICR strain mice for 14 days. Gynura procumbens did not show any toxicity at concentration 7 g/kg b.w after administered orally. These results suggest Gynura procumbens and other herbs contain active constituents and have potentials for diabetes treatment.