Xanthorrhizol Exhibits Antiproliferative Activity on MCF-7 Breast Cancer Via Apoptosis Induction

Author

Yew Hoong Cheah, Hawariah Lope Pihie Azimahtol, and Noor Rain Abdullah

Proceeding

Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia.

Date

17/7/2007

Keyword

Xanthorrhizol, C. xanthorrhiza, antiproliferative activity, MCF-7, apoptosis.

Abstract

Xanthorrhizol is a natural sesquiterpenoid compound isolated from the rhizome ofCurcuma xanthorrhizaRoxb (Zingiberaceae). Xanthorrhizol was tested for a variety of important pharmacological activities including antioxidant and anti-inflammatory activities. An antiproliferation assay using the MTT method indicated that xanthorrhizol inhibited the proliferation of the human breast cancer cell line, MCF-7, with an EC50 value of 1.71 μg/ml. Three parameters including annexin-V binding assay, Hoechst 33258 staining and accumulation of sub-G1 population in DNA histogram confirmed the apoptosis induction in response to xanthorrhizol treatment. Western-blotting revealed down-regulation of the anti-apoptotic bcl-2 protein expression. However, xanthorrhizol did not affect the expression of the pro-apoptotic protein, bax, at a concentration of 1 μg/ml, 2.5 μg/ml, and 5 μg/ml. The level of p53 was greatly increased, whilst PARP-1 was cleaved to 85 kDa subunits, following the treatment with xanthorrhizol at a dose-dependent manner. These results, thereby, suggest that xanthorrhizol has antiproliferative effects on MCF-7 cells by inducing apoptosis through the modulation of bcl-2, p53 and PARP-1 protein levels.