Evaluation of Anticancer Effect and Cell Cycle Alteration of Extract of Pistacia atlantica in Human Breast Cancer T47D Cells Using Flow Cytometry

Author

Shamileh Fouladdel, Mohammadhossein Abdolmohammadi, Yousuf Kamrani, Tina Asgari, Gholamreza Amin and Ebrahim Azizi

Proceeding

Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia.

Date

17/7/2007

Keyword

anticancer, Pistacia, cell cycle

Abstract

Screening for new anticancer drugs with synthetic or plant origin is one of the main focuses of many cancer research laboratories. Iran has unique plant coverage which many of them have not yet been fully studied for their potential anticancer effects. Therefore, we studied the anticancer effects ofPistacia atlantica, an Iranian cultivar of family ofAnacardiaceaewith local name ofBaneh, in human breast cancer T47D cells. The T47D cells were seeded in 96-well culture plates in the presence and absence of different concentrations of fruit-skin extract ofBanehto determine anticancer effects using MTT assay. The cell cycle pattern of T47D cells following exposure toBanehextract was also determined using DAPIin flow cytometric analysis. Extract ofBanehshowed strong growth inhibitory effects on proliferation of T47D cells when compared to RPMI control and Doxorubicin as a known anticancer drug.Banehextract clearly showed alterations in theG1, S, and G2/M proportions of the cytograms compare to control RPMI and Doxorubicin. Our findings are the first data on potential anticancer effect ofBanehextract and one of its possible mechanisms of action on human breast cancer T47D cell proliferation.