Phytochemical Study and Cytotoxicity of<em>Convolvulus arvensis</em>Extracts on Human Cancerous Cell Line

Author

Hojjat Sadeghi-aliabadi, Nasrollah Ghasemi and Mahdokht Kohi

Proceeding

Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia.

Date

17/7/2007

Keyword

Convolvulus arvensis, cytotoxicity, MTT assay

Abstract

There has been a great interest in medicinal plans as a source of new anticancer agent. Some studies have noted thatConvolvulus arvensishas shown cytotoxic effect on some cancerous cell line. Regarding to this, in this study we decided to evaluate the cytotoxic effects ofC. arvensison hela cell line. Arial parts ofC. arvensiswere collected from Isfahan university campus.  It was chopped, dried in shade and primary phytochemical studies were done on plant powder. Hydroalcoholic, chlorformic and ethylacetate extracts were prepared by perculation, reflux and soxhlet, respectively. Then cytotoxic effect of extracts (concentrations from 9.375 μg/ml) against Hela cells were evaluated using colorimetric MTT assay (n=3). Primary phytochemical studies showed the presence of alkaloids, flavonoids, saponines, glycoside and tannines in this plant. Hydroalcoholic, ethylacetate and chlorformic extracts were effective against Hela cells in concentrations of 75 μg/ml, 37.5μg/ml and 18.75 μg/ml respectively (cell survival < 50%). Some studies have revealed that the presence of polar cytotoxiccompounds called proteoglycan mixture in this plant. It seems these compounds can be extracted by polar solvents butthe results showed less cytotoxicity with polar extracts. On the other hand, higher cytotoxic effects of chloroformic extract in inhibition of cell growth indicated that some other components extracted in non polar solvents are responsible for these effects. This need to be more explored by future studies.