The In vivo Rodent Micronucleus Assay of Kacip Fatimah (Labisia pumila) Extract  

Author

Zaizuhana Shahrim, Puteri J. Noor M Baharuddin, Noral ‘Ashikin Yahya, Hussin Muhammad, Rohana A Bakar and Zakiah Ismail

Proceeding

Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia

Date

17/7/2007

Keyword

Bone marrow, genotoxicity,Labisia pumila, micronucleus

Abstract

Kacip Fatimah also known asLabisia pumila(Myrsinaceae), is a traditional herbal medicine with a long history in the Malay community.  It has been used by many generations of Malay women to induce and facilitate childbirth as well as a post­partum medicine. We tested the genotoxic potential of Kacip Fatimah in bone marrow cells obtained from Sprague-Dawleyrats using micronuclei formation as the toxicological endpoints. Five groups of five male rats each were administered orally with doses of 100, 700 and 2000 mg/kg body weight of Kacip Fatimah extract dissolved in distilled water.  Micronucleus preparation was obtained from bone marrow cells of the animals following standard protocols. No statistically significant increase in micronucleated polychromatic erythrocytes (MNPCEs) was observed at any dose level and sacrifice/harvest time point (24, 48 and 72h). However, a significant decrease in polychromatic erythrocytes/normochromatic erythrocytes(PCE:NCE) ratio was observed from the highest dose level (2000 mg/kg of body weight) at 48h harvest time point. In this study, we investigated the effect of Kacip Fatimah on mammalian bone marrow cells using micronuclei formation to assess the genotoxicity of the herb.