Acute and Subacute Study of Eurycoma longifolia (TA164), on Sprague Dawley Rats

Author

Noor Rain A, Ilham, A, Mohd Ridzuan MAR, Ridzhan R, Cheah Y H, Hamdino I, Zhari I. NoralÂ’ Ashikin Y and Zakiah I.

Proceeding

Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia

Date

17/7/2007

Keyword

Eurycoma longifolia, acute toxicity, subacute toxicity

Abstract

Eurycoma longifoliaor locally know as the Tongkat Ali is the most popular medicinal plant in Malaysia. It has many traditional claims, believed to be able to treat a range of chronic diseases to minor ailments and can also be used as health supplements. Currently they are used as supplements in beverages, coffee and in tea.  Some of the traditional claims have been proven scientificallyin vitroand in animal model, such as for treatment for fever (anti malaria) and growth inhibition activities. Some of the preparation of the root extracts and the constituents have been shown to have cytotoxicity effects toin vitrocell lines. Its wide spectrum of pharmacological activities associated with the crude extracts and its constituents have further prompted us to understand its possible effect on systemic toxicology study. The present study is to determine the toxicity effect ofE. longifolia(TA164, MEOH preparations) on Sprague Dawley Rats, through the acute and subacute toxicity study. The study was carried out based on the Malaysian National Guidelines, Ministry of Health, and OECD Guideline For The Testing Of Chemicals (the minimal requirements the laboratory could carry out). The acute toxicity study was according to the OECD guideline 401, 423 while the subacute study was based on OECD Guideline 422. The used of the laboratory animals and its study design were approved by the Institutional Animal Care and Used Committee (IACUC) of Ministry of Health Malaysia. The Acute toxicity study (single dose at 5 g/kg BW) showed that generally, there was no acute effect of TA164 to the rats. However acute toxicity data sometimes is of limited clinical application since accumulative toxic effect may not be seen in short period with a single dose application. Based on this data, we determine the doses for the sub acute study. The doses for sub acute study were 50, 200 mg/kg and 1000 mg/kg BW. TA164 was given daily for 28 days. There is significant different with the changes in body weight of group receiving 1000 mg/kg BW TA  on Week 2, 3 and 4 as compared to the control group. Changes in body weight have important influence on many aspects of animal responses including shift in metabolic, hormonal and hemostatic mechanism. There are parameters in the renal profile and the liver profile that showed a significant different as compared to the croups.