Screening of Extracts and Fractions From Aerial Parts of Stachys schtschegleevii for Anti­-inflammatory Activities

Author

Alireza Garjani, Nasrin Maleki and Hosein Nazemiyeh

Proceeding

Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia

Date

17/7/2007

Keyword

Stachys schtschegleevii, anti-inflammatory, extract

Abstract

Stachys schtschegleeviiis a native plant widely distributed in Iran and belongs to family of Lamiaceae and genus of Stachys.The plant is used in Iranian folk medicine in infective, rheumatic and other inflammatory disorders. In the present studythe anti-inflammatory properties of different extracts and components isolated from aerial parts ofS. schtschegleeviiwere investigated. In this study Thin Layer Chromatographic, Preparative-HPLC, and 1H and 13C-NMR were used for phytochemical analyses. Carrageenan-induced rat paw edema was used as an acute inflammation model. Intraperitoneal injection ofhydroalcoholic extract 60 min before the induction of carrageenan rat paw oedema significantly reduced the maximaledema response attained during 4 h, the total edema response and neutrophil infiltration to the paw. Petroleum, aqueous and n-buthanol extracts caused no significant inhibition of the carrageenan-induced inflammation, whereas chloroformic extract with high doses produced a pro-inflammatory response. At least three flavonoids penduletin, xanthomicrol, and cirsimaritin were identified in chloroform extract. The ethyl acetate extract caused a potent and dose-related inhibition of the inflammation. This extract was fractionated into 11 major fractions according to increasing polarity of solvent mixtures.  The most prominent anti-inflammatory effect was observed with fractions 8 to 10, which contained caffeic acid derivatives, mainly acteoside (3). Thin Layer Chromatographic (TLC) analyses showed the presence of caffeic acid derivatives and flavonoids as the major classes of compounds present in the most of these fractions. Preparative-HPLC and NMR analyses of thesefractions led to the isolation and identification of three major compounds, chrysoeriol 7-O-β-[6”-(p- coumaroyl)]-glucoside (1),apigenin 7-O-β-[6”-(p- coumaroyl)]-glucoside (2), and acteoside (3). These results suggest that the hydroalcoholic extract of aerial parts of attenuate the inflammatory response and the active principal(s) responsible for this effect is likely related to the presence of the phenylethanoid glycosides. This extract also contains pro-inflammatory elements, which are presentedin lipid phase of the extracts.