Prophage Induction in Escherichia coli K-12 by Some Plants From Iran

Author

Farzaneh Naghibi, Mahdieh Taghvaei, Mahmud Mosaddegh and Nasrin Moazzami

Proceeding

Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia

Date

17/7/2007

Keyword

prophage, Escherichia coli K-12, DNA-reactive

Abstract

The test of induction of lambda-phage formation in lysogenicEscherichia coliK-12 (lambda) was used in a study of some medicinal plants from Iran. In this study, 19 plants from North-eastern of Iran (Turkmen Sahra) were chosen based on ethnobotanical information and screened for biologic activity. 100 μg of dried extract dissolved in DMSO were spotted to the surface of the plates includingE.coli, strain K12 (lambda). Mitomycin C is used as positive control to check the efficiency of the experiment. The active extract cause the prophage to be released from the host genome and the phage reverts to the lytic mode and lyses cells. Production of plaque shows this effect. Extract from roots ofFerula szowitsiana, Stachysturcomanica Trautv, Satureja mutica fisch, Echium italicum, Hymnocrater platystegia, Glycyrhiza glabrashowed positive effect. One of the 6 effective plants,Ferula szowitsiana, was chosen for fractionation and further studies. The induction test may provide a useful screen for the detection of potential DNA-reactive agents.