In Vitro Biocompatibility Evaluation of Biodegradable Polymers in Fibroblast Cells

Author

SIEW EE LING, NOR FADILAH RAJAB, ANUAR BIN OSMAN , KUMAR SUDESH AND SALMAAN HUSSAIN INAYAT-HUSSAIN

Proceeding

The 21st Annual Seminar of the Malaysian Natural Product Society.(Herbal Medicine:Natural Products S ciety), Palm Garden, Putrajaya Malaysia.

Date

22/11/2005

Keyword

In Vitro, Biocompatibility, Biodegradable Polymers, Fibroblast Cells, Delftia acidovorans

Abstract

Among the various biomaterials available for tissue engineering and therapeutic applications, microbial polyhydroxyalkanoates (PHAs) offer the most diverse range of thermal and mechanical properties.  Of particular interest are the PHAs that contain 4-hydroxybutyrate, (4HB), such as poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [(P(3HB-co-4HB]). These polyesters can only be synthesized by a few types of bacteria, among which Delftia acidovorans has the most efficient metabolic pathways to regulate 4HB monomers. The resulting polyesters are bioabsorbable and are being developed as new biomaterial for medical application. P(3HB-co-50 mol% 4HB) copolymer was produced by Delftia acidovorans when cultivated on a mixture of glucose and 1,4-butanediol. The interaction of tissue with biomaterials is an area of crucial importance in determining their safety. Cytotoxic evaluation using MTT assay, was carried out on P(3HB-co-50 mol% 4HB) extract in V79 fibroblast cells at the highest concentration, 200 mg/ml with Polyglactin 910 as a reference material. Our results demonstrated that the cytotoxicity of P(3HB-co-4HB) was comparable with the clinically used Polyglactin 910 where more than 60% of the cells were viable following 72 hr treatment with the extract at 200 mg/ml. Further morphological investigation on investigated the mode of cell death using acridine orange/propidium iodide staining found  an increase in apoptotic cells in a time-dependent manner. In conclusion, P(3HB-co-4HB) showed a mild cytotoxic effect  which was comparable to the currently used polymer Polyglactin 910 suggesting the  potential to be used for future medical application after undergoing further biocompatibility assessment.