Toxic and Antioxidant Effects of the Methanol Extract of Plantago major  on Rats

Author

AHMAD ROHI GHAZALI, OOI PHEI YEIK, SHARIFA AZIZ & RAZINAH SHARIF

Proceeding

The 21st Annual Seminar of the Malaysian Natural Product Society.(Herbal Medicine:Natural Products S ciety), Palm Garden, Putrajaya Malaysia.

Date

22/11/2005

Keyword

Plantago major, acute toxic effects, malondialdehyde, superoxide dismutase, histological studies, antioxidant.

Abstract

The aim of this study was to evaluate the toxic and antioxidant effects of the methanol extract of Plantago major on rats in vivo. Twenty four Wistar male  rats  used were divided into control group (n=6) and three treatment groups (n=6). The treatment groups were fed with different doses of  2000, 4000 and 6000 mg/kg, respectively.  The extract was given to the rats by oral route (force feeding) and observed for 2 weeks. No rats died throughout the study. The toxicity study was carried out by histological studies on various organs such as brain, heart, liver and kidney and the antioxidant property was evaluated by determining the level of malondialdehyde (MDA) and the specific activity of enzyme superoxide dismutase (SOD). Histological studies on the organs, stained by hematoxylin and eosin, showed no cytological abnormalities.  The levels of MDA in the plasma and organ of treatment groups were significantly reduced and specific activity of enzyme SOD increased significantly compared to the control group (p<0.05). The results showed that the methanol extract of  P. major has antioxidant properties and did not exhibit any toxic effect  on the  rats.