1,1-Diphenyl-2-picrylhydrazyl hydrate (DPPH) Radical Scavenging Activity and Total Phenolic Content of Some Common Allium spp. Found in Malaysia

Author

CHAN PEK YUE, NURHAYATI ZAINAL ABIDIN & NORHANOM ABDUL WAHAB

Proceeding

The 21st Annual Seminar of the Malaysian Natural Product Society.(Herbal Medicine:Natural Products S ciety), Palm Garden, Putrajaya Malaysia.

Date

22/10/2005

Keyword

Alliums, antioxidant, total phenolic contents, DPPH assay

Abstract

The Allium genus consists of about 700 biennial and perennial herbs species which give a significant pungent odour when crushed. Majority of Alliums are known as wild plants with little economical value and they have been used traditionally and practiced in folk medicine for over 4,000 years. In the present study, the crude petroleum ether, chloroform and methanol extracts of these seven common Alliums found in Malaysia, namely Allium cepa L. (onion), Allium fistulosum L. (spring onions), Allium porrum L. (leek), Allium sativum L. (cultivated garlic), Allium ursinum L. (single-clove garlic) and two variants of Allium tuberosum L. - ‘bunga kucai’ and ‘daun kucai’ - were tested for their antioxidant capacity using the 1,1-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay. The antioxidant potential of each plant was then correlated with their total phenolic contents assayed using the Folin-Ciocalteau method. The antioxidant activity of Allium cepa L. crude chloroform extract as seen using the DPPH radical scavenging assay showed positive correlation with its total phenolic content. However, no such correlation can be made as yet with other Alliums in this study.