Preliminary Investigation on Protective Properties of Fruit Juices on Irradiated Rat Lymphocytes

Author

KHAIRUL, O., RACHEL, G., PROMWICHIT, P., JAMALUDIN, M.

Proceeding

The 21st Annual Seminar of the Malaysian Natural Product Society.(Herbal Medicine:Natural Products S ciety), Palm Garden, Putrajaya Malaysia.

Date

22/11/2005

Keyword

Fruit juice, irradiated rat lymphocytes, gamma radiation, radioprotection.

Abstract

This study was conducted to investigate the ability of selected natural products to protect irradiated rat lymphocytes. Until now it is known that ionizing radiation will split water molecules and there by produce excessive amounts of free radicals. These free radicals will then accumulate and ultimately cause bimolecular damage. Extensive damage at bimolecular level will then be expressed at macroscopic level. Among the first type of cells to express this damage are the lymphocytes. Fresh juices composing of mengkudu (Morinda citrifolia Linn), mango (Mangifera indica), star fruit (Averrhoa carambola), kiwi (Actinidia chinensis), watermelon (Citrullus vulgaris), bananas (Musa paradisiacal and Musa acumiata colla), pegaga (Centella asiatica) and sapodilla (Manikara zapota) were used in this study at 20 g/kg, 30 g/kg and 40 g/kg of animal weight per day. A total of 29 groups composed of negative, positive control and three concentrations of each fruit juice were allocated with each group composed of six adult rats. Gamma radiation of 90 rads was given after a week of force-feeding. After 24 hrs exposure blood smears were done to determine total white blood cells count and population composition. Results indicated that with the exception of star fruit juice, percentage of radioprotection to the lymphocytes was in parallel with the various concentrations. The reductions of lymphocytes were present in all groups, but with the exception of Musa sp juices the reductions were significantly much less than the negative control and much more than the positive control (p<0.05). It was also found that watermelon juice had the best radioprotection properties at 30 g/kg concentrations and above compared to other fruit juices. In conclusion, utilizing selected fruit juice could be used to minimize the sudden reduction of lymphocytes for patients undergoing radiotherapy.