Cytotoxic Activty of Selected Leguminosae Species Against CaSki Cells

Author

MAIZATUL AZMAH, A.L., NURHAYATI, Z.A. & NORHANOM, A.W.

Proceeding

The 21st Annual Seminar of the Malaysian Natural Product Society.(Herbal Medicine:Natural Products S ciety), Palm Garden, Putrajaya Malaysia.

Date

22/11/2005

Keyword

Leguminosae species, Pisum sativum, Phaseolus vulgaris, Vigna sinensis, Sesbania grandiflora, cytotoxic activity, CaSki cells.

Abstract

In this study the aqueous and methanol extracts of four Leguminosae species, i.e. Pisum sativum, Phaseolus vulgaris, Vigna sinensis, and Sesbania grandiflora were screened for their cytotoxic potential against a cervical cancer cell lines, CaSki, by an in vitro assay system of growth inhibition. The CaSki cells maintained in 10% RPMI 1640 were treated separately with each of the crude extracts at various concentrations of 0, 1.0, 10.0, 25.0, 50.0, and 100.0 µg/ml for 72 hours at 37°C in 5% CO2. The Neutral Red Cytotoxic Assay was then carried out and the IC50 value for each extract was determined.  The extracts with IC50 values of less than 20 µg/ml were considered active. The methanol extracts of P. sativum, P. vulgaris, and V. sinensis were active against CaSki cells with IC50 values of 14.8, 15.0, and 19.8 µg/ml, respectively. The water extracts of P. vulgaris and V. sinensis were active with IC50 values of 6.0 and 19.0 µg/ml, respectively. However, the remaining extracts were not active. The study suggested that the Leguminosae species may possess potential anticancer properties. Thus, further investigations should be carried out to identify the bioactive principles contained therein.