Genotoxicity Evaluation of the Ethanolic Extract of Solanum torvum Fruit on Peripheral Lymphocytes

Author

SITI SARAH UDZIR*, NOR FADILAH RAJAB, ENGKU NORBAYA ENGKU MUDA, AHMAD ROHI GHAZALI, AZMAN ABU BAKAR & PADEN MORAT

Proceeding

The 21st Annual Seminar of the Malaysian Natural Product Society.(Herbal Medicine:Natural Products S ciety), Palm Garden, Putrajaya Malaysia.

Date

22/11/2005

Keyword

Solanum torvum, genotoxicity evaluation, peripheral lymphocytes.

Abstract

Solanum torvumhas long been used traditionally to treat various diseases. In vivo studies have shown it effects in lowering blood lipid. This study evaluates the genotoxic potential of ethanolic extract ofS. torvumfruit on peripheral lymphocytes. Two groups of male Hartley guinea pigs were fed either normal or high-cholesterol (2%) diet for six weeks. Simultaneously, each group was either received no treatment, 50 mg/kg body wt. of S. torvum extract or 10 mg/kg body wt. of atorvastatin daily.  Genotoxicity evaluation was carried out using Alkaline Comet assay. Percentage of damage to the DNA was evaluated using software based on the tail moment and tail intensity values and classified into different score accordingly.S. torvum-treated lymphocytes showed far less DNA damage as compared to the untreated lymphocytes. Similar result was seen in atorvastatin-treated lymphocytes. On the contrary, lymphocytes from high-cholesterol animals showed severe levels of DNA damage. In conclusion,S. torvumextract showed a protective effect on the damage of the DNA of peripheral lymphocytes.