Tissue Culture Of Roses

Author

Kong, S. L. Marziah, M. And Norlela K

Proceeding

The Nineteenth Malaysian Biochemical & Molecular Biology Society Conference : Challenges Of Biochemistry Into The 21st. Century, Faculty Of Medicine , Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

Date

31/10/1994

Keyword

tissue culture , roses

Abstract

An attempt is being carried out to establish a tissue culture system for direct and indirect regeneration of roses (Rosa sp). These studies are important as to obtain a protocol to regenerate transformed rose tissues to obtain a transgenic rose plant. In this study various explants were used to initiate callus. Calli were initiated from leaves and stems in MS basal media (Murashige and Skoog, 1962) with 30 g/L sucrose and B5 (Gamborg et at, 1968) vitamins supplemented with 2 mg/L 2,4-D + 1 mg/L kinetin. Calli formed from the cut edges of leaves and stems after 7 to 10 days in culture. Calli were also Initiated from rose petals in MS basal media with 1 mg/L NAA + I mg/L kinetin. Calli from rose petals resulted in red pigmentation. In order to select for the best pigmentation from rose petals, calli were also cultured in MS basal media with B 5 vitamins and 30 g/L sucrose together with different concentration of BAP and Kinetin. For direct regeneration, axilllary buds were cultured in MS basal media with three different concentration of BAP (0.5, 1.0, and 2.0 mg/L). Shoots formed in all the media tested. Studies are still on going to select for the best regeneration protocol.