Brine Shrimp Lethality Assay: Potentiation Of Cyclophosphamide On The Cytotoxicity Activity Of Kaempferia galanga

Author

Abas Hj Hussin, Pazilah Ibrahim, Amirin Sadikun and Mariam Ahmad, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.

Proceeding

Trends in Traditional Medicine Research, Proceedings of the International Conference on the Use of Traditional Medicine & Other Natural Products in Health Care

Date

8/6/1993

Keyword

Kaempferia galanga. Brine Shrimp Lethality Assay, cyclophosphamide

Abstract

The cytotoxic activity of K. galanga and its interaction with cyclophosphamide was investigated using the brine shrimp lethality assay. Chloroform extract of K. galanga, ethyl-p-methoxycinnamate and propyl-p-methoxycinnamate exhibited significant cytotoxic activity (P< 0.001) when compared to their controls ai concentration as low as 0.1 µg/ml, 10 µg/ml and 100 µg/ml respectively. Cyclophosphamide was found to show no cytotoxic activity at concentrations in the range of 0.1 - 1000 µg/ml. However, the activity of the chloroform extract of K. galanga, ethyl-p-methoxycinnamate and propyl-p-methoxycinnamate were significantly potentiated in the presence of cyclophosphamide. Our results indicated that the chloroform extract of K. galanga exhibited the most potent cytotoxic activity among the three and their action could be potentiated in the presence of cyclophosphamide.