Protective Effect Of Gypenosides On Free Radicals Induced Damage Of Isolated Guinea Pig Papillary Muscles And Rabbit Arterial Endothelium

Author

Liao DF, Qiu LB * , Xiao GL, Chen JX, Yu L and Cao JG Department of Pharmacology and* Physiology, Hengyang Medical College, Hengyang 421001, Hunan,P. R.China

Proceeding

Trends in Traditional Medicine Research, Proceedings of the International Conference on the Use of Traditional Medicine & Other Natural Products in Health Care

Date

8/6/1993

Keyword

Gypenosides, papillary muscle, endothelium-derived relaxing factor, basial arteries, guinea pig, rabbit.

Abstract

We report here the protective effect of gypenosides (GPS) against damage due to OFR on guinea pig myocardium and rabbit arterial endothelium. In the isolated guinea pig papillary muscles, GPS 50 ug/ml significantly reversed X-XOD-induced negative inotropism, prolongated FRP and reduced the automaticity and excitability. At the same time, GPS markedly antagonized OFR-induced rise of MDA and decrease of SOD. In isolated rabbit thoracic aortas, GPS 25, 50 and 100 ug/ml significantly protected the EDRF-release capacity from OFR damage in a concentration-dependent manner. GPS 100 ng/ml also prevented the rise of perfusion pressure by OFR in the isolated rabbit basial arteries.  It has recently been reported that gypenosides (GPS) isolated from Gynostemmapentaphyllum (Thunb) Makino, could lower myocardiac oxygen consumption in anesthetized dogs [1]. They also could reduce myocardial infarct size produced by occlusion of left anterior decending coronary artery in rabbit [2], protect against ischemic myocardial damage |3] and inhibit the lipid peroxidation of liver in rat |4], which implicate that GPS may possess antioxidant action. The present work investigated whether GPS can protect myocardium and aortic endothelium from damage directly produced by exogenous oxygen free radicals.