Effects Of Celery On Serum Cholestrol And Triglyceride Levels In High Fat Diet-Fed Winstar Rats

Author

D. Tsi. N.P Das*, A. Hsu and B.K.H. Tan, D. Tsi. N.P Das*, A. Hsu and B.K.H. Tan, Faculty of Medicine, National University of Singapore 10 Kent Ridge Crescent, Singapore 0511

Proceeding

Trends in Traditional Medicine Research, Proceedings of the International Conference on the Use of Traditional Medicine & Other Natural Products in Health Care

Date

8/6/1993

Keyword

Celery, Apium graveolens, lipoproteins, triglyceride

Abstract

When aqueous extract of celery [Apium graveolens L. var dulca (Miller) Pers. (Umbilliferae)] was given by intraperitoneal infusion for 13 days in genetically hypercholesterolemic rats, serum cholesterol but not triglyceride concentration was lowered significantly. We decided to study the effects of an 8-week oral intake of celery extract on the serum lipid levels of Wistar rats fed on a high fat diet. An aqueous extract of celery was made by blending 3 kg of stems and leaves in 6 liters of deionized water. The extract was filtered and concentrated by distillation before being lyophilized into a yellow powder. From the weight of powder obtained, the extraction ratio was calculated. Celery powder equivalent in amount to 1/2 stalk of celery was fed to six male Wistar rats given a high fat diet (HFD). Six other rats were given high fat diet without the celery powder and serve as controls. After 8 weeks, the rats were killed and serum was obtained for the estimation of total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), and triglyceride (TG) concentration using diagnostic kits from Boehringer-Mannheim. Low density lipoprotein cholesterol (LDL-C) concentration were calculated using the Friedewald Formula {LDL-C -TC-(HDL-C)-(TG/5)}. Statistical analysis was carried out by Student's t-test for p<0.05. The mean serum TC and TG levels in the celery-treated rats were significantly reduced by 34 % and 42 % respectively as compared to the controls. While the mean serum HDL-C levels remained similar in both groups, mean serum LDL-C concentration in the celery-treated rats showed a significant reduction of 42 % when compared to the control group. The anti-atherogenic index (HDL-C/TC-[HDL-C]) calculated was found to be 21.7 % higher in the celery-treated rats than the controls. Decreases in the mean serum TC, HDL-C, LDL-C and TG levels of the celery-treated rats compared to the controls are as follows: Δ TC (mg %) ΔHDL-C (mg %) Δ LDL-C (mg %) Δ TG(mg%) -50.1* -4.0 -3.25* -68.4* * p<0.05 In conclusion, chronic treatment with celery extract in the diet significantly reduced serum TC. LDL-C and TG levels in high fat diet-fed Wistar rats.