Strobilanthes crispus Tea And Women's Cancer Cell Lines

Author

Asmah Rahmat1, Arnida Hani Teh1, Fauziah Othman2, and Normah Hashim1. 1Department of Nutrition and Health Sciences, 2Department of Biomedical Sciences, Faculty of medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Proceeding

1st International Conference & Exhibition on Women's Health & Asian Traditional (WHAT) Medicine

Date

23/8/2005

Keyword

Strobilanthes crispus , Acanthaceae, herbal tea, antiproliferative effects, cell lines

Abstract

Strobilanthes crispus ZII 109 (L) Bremek or Saricocalix crispus ZII 109 (L) Bremek (Acanthaceae) plant is native to countries from Madagascar to Indonesia.  Nowadays, more variation of teas were produced using this plant and marketed. In addition to Camellia sinensis tea, now teas are made from other plants especially herbal. Since interest has now moved to other ‘teas’, several experimental tests were carried out in order to fulfill these objectives: to develop fermented and unfermented teas of Strobilanthes crispus leaves, to evaluate the sensory qualities, and to determine the antiproliferative effects of the prepared samples on several human cancer cell lines, i.e. hormone-dependent breast cancer (MCF-7), ovarian carcinoma (CAOV3) and human cervical carcinoma cell lines (HeLa). Generally, four different products were developed in this study; fermented Strobilanthes crispus tea (young leaves), fermented Strobilanthes crispus tea (old leaves), unfermented Strobilanthes crispus tea (young leaves), and unfermented Strobilanthes crispus tea (old leaves). Results from the antiproliferative studies showed that; the fermented Strobilanthes crispus tea (old leaves) methanol extract, fermented Strobilanthes crispus tea (old leaves) water extract, unfermented Strobilanthes crispus tea (old leaves) methanol extract and unfermented Strobilanthes crispus tea (old leaves) water extract inhibited 50 % of the MCF-7 cell growth with IC50 values = 23.0, 72.5, 63.0 and 80.5 g/ml respectively, the unfermented Strobilanthes crispus tea (old leaves) methanol extract, unfermented Strobilanthes crispus tea (old leaves) water extract, fermented Strobilanthes crispus tea (young leaves) water extract, and fermented Strobilanthes crispus tea (old leaves) methanol extract, inhibited 50 % of the CAOV3 cell growth with IC50 values = 54.12, 57.22, 13.91 and 67.39 ¿g/ml respectively, while unfermented Strobilanthes crispus tea (old leaves) methanol extract and unfermented Strobilanthes crispus tea (old leaves) water extract inhibited 50% of the HeLa cell growth with IC50 values = 99.38 and 23.33 g/ml respectively. On the other hand, sensory evaluation showed that the overall acceptability score was found highest for unfermented Strobilanthes crispus tea (old leaves), and this positively related with its ability to inhibit 50% of the proliferation of hormone-dependent breast cancer (MCF-7), ovarian carcinoma cell lines (CAOV3) and human cervical carcinoma cell lines (HeLa) with IC50 values less than 100 g/ml.  Generally, all the tea infusions were satisfactorily acceptable. These teas may present a potential use as an herbal tea worth promoting for commercialization.