Investigation Of Biological And Chemical Differences Between Mature And Immature Tubers Of Ipomoea mauritiana

Author

A.Shanthi, V.B.Preethi sudha, S.Lalitha and P.Venkatasubramanian*, Foundation for Revitalisation of Local Health Traditions (FRLHT), Jarakabande Kaval, Attur post via YelahankaBangalore – 560064, India

Proceeding

1st International Conference & Exhibition on Women's Health & Asian Traditional (WHAT) Medicine

Date

23/8/2005

Keyword

Ayurveda, herbal formulations, Ipomoea mauritiana, enhance lactation, biological and chemical differences

Abstract

Ayurveda is a traditional health system of India with its origin firmly rooted in Indian subcontinent, but its popularity increasing all over the world. In Ayurveda, an experienced traditional practitioner guides the preparation of herbal formulations. The efficacy of the formulation is dependent on the quality of raw drug ingredients, which in turn is determined by factors such as time and region of collection and method of processing. It is not sufficient to just use a raw drug of the correct botanical identity; it is equally important to use a raw drug collected at the right stage of maturity for it to have best potency. Ipomoea mauritiana (Jacq.) (Vidari- Sanskrit) is used in formulations to enhance lactation in nursing mothers. Only the mature tubers of the plant are used in traditional preparations. Immature tubers are discarded. The aim of the current study was to investigate the biological and chemical differences between mature and immature tubers. The mature tuber of Ipomoea mauritiana (Jacq.) was compared with the immature tuber to find out the differences in their phytoconstituents and their bioactivities. The chemical profiles of these tubers were distinguished using HPTLC and their biological activity was compared using Brine Shrimp Bioassay. Brine shrimp assay has been used in recent times to determine biological activity of medicinal plants. Brine shrimps are crustaceans that are extremely sensitive to their external environment so much so that this makes them useful in toxicity testing and screening of medicinal plants for biological activity. The procedure determines LC50, which is the concentration of plant extract that kills 50% of the shrimp nauplii used within 24hrs of contact with the extract. The bioactivity of the extracts is inversely proportional to the LC50 value. The method is rapid, reliable, inexpensive and convenient as an in-house general bioassay tool. The chemical and biological profiles showed that mature tuber had quantitatively and qualitatively more phytoconstituents and its biological activity was twice that of the immature tuber. Our further work is extended in isolating the phytoconstituent, which is distinctly present in mature tuber, and in characterising it. This phytoconstituent may be used in future for distinguishing mature tuber from the immature one and will be a relevant reference marker that is linked to efficacy.