Effect of Pressure and Temperature of Supercritical Carbon Dioxide on the Antioxidant Compounds and

Author

DR MOHD AZIZI CHE YUNUS
*1C. Y. M.  Azizi, and 2 Ramlan Abdul Aziz
1 Centre of Lipids for Engineering Applied Research, CLEAR, Chemical Engineering Department, Faculty of Chemical and Natural Resources Engineering, Universiti Teknologi Malaysia, 81310 Skudai Johor.
2 Chemical Engineering pilot Plant, CEPP, Universiti Teknologi Malaysia, 81310 Skudai Johor

Language

English

Title

Effect of Pressure and Temperature of Supercritical Carbon Dioxide on the Antioxidant Compounds and Extract Naturalness of Pithecellobium jiringan jack seeds (Malay Ulam Jering)

Proceeding

7th INTRACOM – 2nd ICBWI 2009

Date

23th July 2009

Place Held

PWTC, Kuala Lumpur

Abstract

A supercritical carbon dioxide extraction condition has been studied as an alternative process for extraction of antioxidant compounds (phenolics and tocopherol) and investigation of `extract naturalness’ of Pithecellobium Jiringan Jack (Prain) seeds. The extract naturalness was referred to high concentration of antioxidant content and their antioxidant activities. Supercritical conditions were temperatures of 40o, 50o, 60o, 70o and 80o C and pressures of 20.68, 27.56, 34.47, 41.37, 48.26 and 55.16 MPa. Antioxidant activities of the extract were determined by DPPH (1, 2-diphenyl-2-picrylhydrazyl) radical scavenging assay. Method was developed for quantification of phenolics and tocopherol compound using Gas Chromatography Time of Flight Mass Spectrometry, GC-TOFMS.  The study shows that the maximum overall extraction yield was 8.06 %, at the highest pressure and temperature of 55.16 MPa and 80o C, respectively. On the other hand, the minimum yield was 1.76 wt % was obtained at lowest pressure and temperature of 20.68 MPa and 40o C, respectively. The concentration of phenolics and tocopherol were increased with decrease in temperature for constant pressure. The maximum concentration of tocopherol is about 0.246 wt % (2.46 mg/ g oil) and was obtained at 20.68 MPa and 40o C, while the antioxidant properties (87%) were high at extraction condition where the extraction yield was low (20.68 – 34.47 MPa and 40 – 60oC). The tocopherol yield under these conditions corresponded to almost complete recovery.

Keywords

Pithecellobium jiringan jack seeds

Session

Symposium 1B (ICBWI)

Topic

Natural & Safe Food Product