Pre-Treatment Study of Asymmetric Ultrafiltration Membrane for Lysozyme Separation

Author

ASSOC PROF DR NORA’AINI ALI
Nora’aini Ali*, H.  Sofiah. M. T. S.Syaima
Department of Engineering Science, Faculty of Science and Technology
University Malaysia Terengganu, 21030 Kuala Terengganu, Malaysia
Tel. +60(9) 668-3254; Fax +60(9) 669-4660; email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Pre-Treatment Study of Asymmetric Ultrafiltration Membrane for Lysozyme Separation

Proceeding

7th INTRACOM – 2nd ICBWI 2009

Date

24th July 2009

Place Held

PWTC, Kuala Lumpur

Abstract

Membrane fouling during ultrafiltration process is critical and complicated phenomenon for demanding application such as protein fractionation since the transportation of solute through the membrane does not depends only on size, but also strongly depends on several factors such as solute-solute and solute-membrane interactions.  This research aimed to modify the asymmetric ultrafiltration membrane via protein adsorption for lysozyme separation in order to get low binding membranes which consequently reduce the fouling during ultrafiltration process.   A good performance of UF15 membrane which has been developed in the previous study was used as a native membrane and modified using adsorption of myoglobin. This pre-treated membrane was characterized in term of pure water permeability, membrane morphology, Molecular Weight Cut Off (MWCO) and membrane surface charge to ensure its suitability for lysozyme purification. Permeability coefficient for the prepared membrane was in the range of UF membrane; for about 1.8 x 10-5 m3/m2.s.bar and its morphology shows an asymmetric structure with large finger like and macrovoid structures.  This pre-treated membrane was negatively charged and performed Molecular Weight Cut Off for about 28kD which is smaller than the native membrane. A promising result was obtained when this membrane was successfully separate the single solution of protein lysozyme with 96% transmission and 5.6 x 10-5 m3/m2.s flux.  This proved the pre-treated membrane could improve the flux and separation performance of protein lysozyme up to 10% and reduce the membrane fouling. It is hope that the findings of this research will provide a window of operation to produce a low fouling membrane for protein separation.

Keywords

Biotechnology, pre-treated membrane, ultrafiltration, lysozyme, myoglobin

Session

Symposium 2B (ICBWI)

Topic

Functional Food & Nutraceutical