Metabolomics as a Tool for Monitoring Health and Disease

Author

DR JULIAN L. GRIFFIN
Julian L. Griffin
Department of Biochemistry, University of Cambridge, Cambridge, UK

Language

English

Title

Metabolomics as a Tool for Monitoring Health and Disease

Proceeding

7th INTRACOM – 2nd ICBWI 2009

Date

24th July 2009

Place Held

PWTC, Kuala Lumpur

Abstract

The analytical techniques used to profile metabolism in tissues and biofluids are relatively cheap on a per sample basis, and thus allow one to profile a range of tissues building up a metabolomic model of the perturbations induced in systemic metabolism. In conjunction with multivariate statistics we have been using a combination of high resolution 1H NMR spectroscopy, Gas Chromatography Mass Spectrometry (GC-MS) and Liquid Chromatography Mass Spectrometry (LC-MS) to profile a range of metabolites in key affected organs in rodent models of disease. This will be illustrated by two examples. In the first the role of the nuclear receptor peroxisome proliferator-activated receptor-alpha (PPAR-α) in regulating systemic metabolism has been followed in an ageing study of the PPAR-α knock out mouse. In this study hepatic steatosis increased in both wildtype and mutant mice with age, with this effect being magnified by age. The second study is an investigation of the action of a PPAR-γ agonist and how metabolomics may be used to identify biomarkers of response in an animal model of type II diabetes/obesity. In this manner metabolomics may be used to determine both biomarkers of response and health in animal models, and ultimately these may be applicable to human disease.

Keywords

Metabolomics

Session

Symposium 2C (ICBWI)

Topic

Biomolecule Discovery