Anti-Obesity Effects of Tropical Herbs

Author

Azizah A.H., Najla G.S., & Nazamid S.    Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor.  Tel: 03-89488374 Fax: 03-89423552 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.  Kitts D. & Purnama M.   Faculty of Food and Land Systems, Food Nutrition and Health, University of British Columbia, 219-2205 East Mall Vancouver, BC, Canada V6T 1Z4

Language

English

Title

Anti-Obesity Effects of Tropical Herbs

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2008)

Date

21st October - 22nd October 2008

Place Held

The Legend Hotel, Kuala Lumpur

Abstract

The effect of Morinda charantia fruit extract (MCE) and Centella asiatica extract (CAE) Morinda citrifolia Fruit Extract (MCFE) on fat metabolism enzyme, lipoprotein lipase (LPL), and on the proliferation and differentiation of 3T3-L1 preadipocytes was investigated to evaluate their potential application for anti-obesity. Dried plant samples were extracted in 99.5% ethanol and the inhibitory effects of the extracts on lipoprotein lipase were determined in vitro. The extent of cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the degree of differentiation was measured using Oil Red O staining assay. All three extracts inhibited LPL activity differently at 1 mg/ml, with MCFE showing the highest inhibitory effect (21.4±2.3%), followed  by CAE (16.3±1.1%) and MCE (10.6±0.6%). On the other hand, MCE showed the highest inhibitory effect on both cell proliferation and differntiation. After 72 h, MCE was most toxic with LC50 of 1.6 mg/L, followed by CAE with 2.4 mg/ml and MCFE had the latest cytotoxic effect with 4.5 mg/ml. After 48 h of plating, MCE had the highest inhibitory effect on cell differentiation in a dose dependent manner. The highest inhibition, 74.7 ± 5.41% of control, occured at concentration 0.5 mg/ml. Our data therefore indicated that of the three extracts, MCE could exert potential anti-obesity effects by inhibiting cell proliferation and diffferentiation of preadipocytes while MCFE could be used as a therapeutic treatment for obesity due to its relatively higher anti-LPL activity.

Keywords

Momordica charantia; Centella asiatica; Morinda citrifolia; plant extracts; anti-obesity assays

Session

Plenary: Session 3

Topic

Think Global, Act Local: A New Paradigm In Malaysian Herbal Industry (Prof. Emeritus Dr. Abdul Latif Ibrahim-UNISEL)