Antioxidant and hepatoprotective actions of medicinal herb, Terminalia catappa L from Okinawa Island and its tannin corilagin

Author

Kinoshita, S., Inoue, Y., Nakama, S., Ichiba, T., Aniya, Y.

Journal

Phytomedicine

Volume

14

Issue ID

11

Page

755-62

Date

2007 Nov.

Keyword

Alternative medicine
Animals
Antioxidants/administration & dosage
Antioxidants/pharmacology*
Antioxidants/therapeutic use
Free Radicals/chemistry
Galactosamine/diagnostic use
Glucosides/administration & dosage
Glucosides/pharmacology
Glucosides/therapeutic use
Hepatitis, Toxic/blood
Hepatitis, Toxic/etiology
Hepatitis, Toxic/prevention & control
Lipopolysaccharides/diagnostic use
Liver/drug effects
Liver/enzymology
Male
Phytotherapy*
Plant Extracts/administration & dosage
Plant Extracts/pharmacology*
Plant Extracts/therapeutic use
Plant Leaves
Protective Agents/administration & dosage
Protective Agents/pharmacology*
Protective Agents/therapeutic use
Rats
Rats, Sprague-Dawley
Tannins/administration & dosage
Tannins/pharmacology
Tannins/therapeutic use
Terminalia*

Abstract

The antioxidant and hepatoprotective actions of Terminalia catappa L. collected from Okinawa Island were evaluated in vitro and in vivo using leaves extract and  isolated antioxidants. A water extract of the leaves of T. catappa showed a strong radical scavenging action for 1,1-diphenyl-2-picrylhydrazyl and superoxide (O(2)(.-)) anion. Chebulagic acid and corilagin were isolated as the active components from T. catappa. Both antioxidants showed a strong scavenging action for O(2)(.-) and peroxyl radicals and also inhibited reactive oxygen species production from leukocytes stimulated by phorbol-12-myristate acetate. Galactosamine (GalN, 600 mg/kg, s.c.,) and lipopolysaccharide (LPS, 0.5 microg/kg, i.p.)-induced hepatotoxicity of rats as seen by an elevation of serum  alanine aminotransferase, aspartate aminotransferase and glutathione S-transferase (GST) activities was significantly reduced when the herb extract or corilagin was given intraperitoneally to rats prior to GalN/LPS treatment. Increase of free radical formation and lipid peroxidation in mitochondria caused  by GalN/LPS treatment were also decreased by pretreatment with the herb/corilagin. In addition, apoptotic events such as DNA fragmentation and the increase in caspase-3 activity in the liver observed with GalN/LPS treatment were prevented by the pretreatment with the herb/corilagin. These results show that the extract of T. catappa and its antioxidant, corilagin are protective against GalN/LPS-induced liver injury through suppression of oxidative stress and apoptosis.

Language

Eng