Pharmacological Evidence for The Activation Of Peripheral Atp-Sensitive K+ Channels By Methanol Extract of Mitragyna Speciosa Korth. In The Formalin Test

Author

Shaik Mossadeg W.M., Syamimi K., Azyyati M.P., Md Lip J., Taufik Hidayat M. & Sulaiman M.R.
Department of Biomedical Sciences, Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor
Laboratory of Cell Culture, Bioassay and Drug Mechanism, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor
Technical Services Centre, MARDI, P.O. Box 12301, 50774 Kuala Lumpur
Tel: 03-89472603/2604 Fax: 03-8947 2585 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Pharmacological Evidence for The Activation Of Peripheral Atp-Sensitive K+ Channels By Methanol Extract
of Mitragyna Speciosa Korth. In The Formalin Test

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2008)

Date

23rd October - 22nd October 2008

Place Held

The Legend Hotel, Kuala Lumpur

Abstract

The possible activation of peripheral ATP-sensitive K+ channels by the methanol extract of Mitragyna speciosa Korth. (MSME) was assessed in the rat 1% formalin test. MSME given by intra-peritoneal route produced significant (p<0.05) dose-dependent anti-nociception in the first and second phases of the test. However, local pre-treatment of the paw with glibenclamide (an ATP-sensitive K+ channel inhibitor), tetraethylammonium (blocker of voltage-dependent K+ channel) and apamin (inhibitor of small-conductance Ca2+ -activated K+ channel) significantly reversed the MSME-induced anti-nociception. These results suggested that the anti-nociceptive effect of MSME was mediated through the activation of ATP-sensitive K channels in addition to the opening of small-conductance Ca2+ -activated K+ channels at peripheral afferent neurons.

Keywords

ATP-Sensitivc K+ Channels; Mitragyna speciosa Korth.; formalin test

Session

Poster Abstracts

Topic

Global, Act Local: A New Paradigm In Malaysian Herbal Industry (Prof. Emeritus Dr. Abdul Latif Ibrahim-UNISEL)