Establishment and Maintenance of Growth Parameters of Cell Suspension Cultures of Ficus Deltoidea

Author

Ong S.L., Ling P.K.A. & Ramani P.
Department of Bioscience,  Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, Setapak 53300, Selangor
Tel: 03-41079802 ext 132 Fax: 03-41079803 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Establishment and Maintenance of Growth Parameters of Cell Suspension Cultures of Ficus Deltoidea

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2008)

Date

24th October - 22nd October 2008

Place Held

The Legend Hotel, Kuala Lumpur

Abstract

Callus derived from the leaf of Ficus deltoidea (mas cotek) was used to establish cell suspension culture in the shake flask system. Different concentrations of plant growth regulators were studied in order to produce rapid-growing and well-dispersed suspension culture of F. deltoidea. Establishment of suspension culture were examined in full strength Murashige and Skoog’s (MS) media supplemented with 3% sucrose and plant growth regulators, either 4-amino-3,5,6-trichloro-2-pyridine (picloram), 2,4-dichlorophenoxy acetic acid (2,4-D) or Dicamba at the concentrations of 1 to 5 mg/I. Packed cell volume (PCV) as well as cell number were measured at every three day interval. Results obtained revealed that MS medium supplemented with 3 mg/I picloram was shown to be the most efficient for the establishment of cell suspension culture giving the highest value of PCV and total cell count. The cell suspension culture of F. deltoidea was successfully established and maintained in this study. The growth curve constructed based on the cell dry weight showed that the culture was in their lag phase between day 0 to day 9. After day 9, the cells eventually entered the exponential phase and almost reaching stationary phase after day 21.

Keywords

Ficus deltoidea; mas cotek; growth parameters; plant cell culture

Session

Poster Abstracts

Topic

Local: A New Paradigm In Malaysian Herbal Industry (Prof. Emeritus Dr. Abdul Latif Ibrahim-UNISEL)