Chemical Fingerprint of Piper Muricatum Blume by Fourier Transform Infrared Spectroscopy (Ftir) With Two Dimensional Correlation Ir Spectroscopy and High Performance Liquid Chromatography (Hplc)

Author

Fauziah, A., Salbiah, M. & Ling. S.K.
Medicinal Plants Programme, Forest Biotechnology Division, Forest Research Institute Malaysia (FRIM), 52109
Kepong, Selangor
Tel: 03-62797671 Fax: 03-62729805 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Chemical Fingerprint of Piper Muricatum Blume by Fourier Transform Infrared Spectroscopy (Ftir) With Two Dimensional Correlation Ir Spectroscopy and High Performance Liquid Chromatography (Hplc)

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2008)

Date

21st October - 22nd October 2008

Place Held

The Legend Hotel, Kuala Lumpur

Abstract

Piper muricatum Blume of the family Piperaceae, locally known as sireh hutan, is a perennial climber and reported as one of the wild Piper species found in the Malaysian forests. Preliminary biological studies demonstrated that methanol extracts of its leaves and stems exhibited therapeutic properties such as anti-microbial, anti-diabetic, anti-inflammatory as well as anti-oxidant. Fourier transform infrared spectroscopy (FT-IR) associated with second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy (2D-lR) were used to study the macroscopic fingerprint of raw materials (stems and leaves) of P. muricatum. The macroscopical fingerprints characters of FT-IR and 2D-IR spectra had indicated many characteristic and holistic variation rules of chemical constituents of the stem and leaves. These were used to identify the main chemical constituents in this plant. This analytical method is highly rapid, effective, visual and accurate for medicinal plant research. This study also attempts to develop HPLC fingerprint of stems and leaves of P. muricatum. Two distinct peaks with UV spectra detected at wavelength 204nm and 284nm were observed in the methanol extract of the leaves whilst one distinct peak with UV spectra at wavelength 204nm and 284nm was observed in the stem. These compounds would be further characterized and could be used as a marker for standardisation for this species.

Keywords

Piper muricatum Blume; FT-lR; second derivative infrared spectroscopy; 2D-IR; macroscopic fingerprint

Session

Poster Abstracts

Topic

Think Global, Act Local: A New Paradigm In Malaysian Herbal Industry (Prof. Emeritus Dr. Abdul Latif Ibrahim-UNISEL)