Evaluation of Anti-Inflammatory and Cytotoxic Activities of Stem and Leaf Extracts Of Plant from the Family Of Thymela Eacaea

Author

Ihsan Safwan K., s1azura M.P., Fadzureena J. & Khoo G.H.M.
Medicinal Plants Programme, Forest Biotechnology Division. Forest Reseach Institute Malaysia, 52109 Kepong.
Selangor
Tel: 03-62797334 Fax: 03-62729805 Email This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Evaluation of Anti-Inflammatory and Cytotoxic Activities of Stem and Leaf Extracts Of Plant from the Family Of Thymela Eacaea

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2008)

Date

21st October - 22nd October 2008

Place Held

The Legend Hotel, Kuala Lumpur

Abstract

DT081540 (stem) and DTO8/541 (leafl are crude extracts of a plant from the family Thymelaeaceae. This plant is traditionally used in aroma-therapy. The present study sought to evaluate the efficacy and safety of these extracts. Methanol extracts of this plant were evaluated for anti-inflammatory activity based on hyaluronidase. lipoxygenase and xanthine oxidase inhibitory assays. The leaf extract (DTO8/541) showed strong inhibition on hyaluronidase. lipoxygenase and xanthine oxidase with inhibition of 96.42 ± 4.08, 81.34 t 0.01 and 84.13 0.01 %. respectively. However, the stem extract exhibited low activity in all assays tested. Cytotoxic effect of the extracts was Investigated in vitro using Sulforhodamine B (SRB) assay on normal kidney (Vero) and normal liver (WRL-68) cell lines. The results showed that the median inhibitory concentration (lC) of stem and leaf extracts in both cell Lines ranged from 60 pg/mI to 415 pg/mi. Since both extracts showed a lC, value above 50 g/ml. it could be considered as non-cytotoxic. This study showed that the ‘eaf extracts of this plant could be further explored for potential usage as remedies for inflammation treatment.

Keywords

Thymelaeaceae; anti-inflammatory activity: cytotoxic activity

Session

Poster Abstracts

Topic

Think Global, Act Local: A New Paradigm In Malaysian Herbal Industry (Prof. Emeritus Dr. Abdul Latif Ibrahim-UNISEL)