Mutagenicity and Anti-Mutagenicity Potentials of Fruit Juices of Five Medicinal Plants in Allium Cepa L.: Possible Influence of Dpph Free Radical Scavengers

Author

Akinboro A., Mohamed K.B., Zaini A., Othman A.S.
School of Biological Sciences, Univerisiti Sains Malaysia, 11800,  Pulau Pinang. Malaysia .
School of Pharmaceutical Sciences. Universiti Sains Malaysia, 11800, Pulau Pinang.
Malaysia
Department of Pure and Applied Biology, Ladoke Akintola University of Technology, P.M. B.
4000, Ogbomoso,  Nigeria
Tel: +60174294512 E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Mutagenicity and Anti-Mutagenicity Potentials of Fruit Juices of Five Medicinal Plants in Allium Cepa L.: Possible Influence of Dpph Free Radical Scavengers

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

This study investigated mutagenic and anti-mutagenic potentials of freeze dried fruit juices of Morinda elliptica Ridl. Morinda citrifolia L., Averrhoa bilimbi L., Phyllantus acidus (L.) Skeels and Myristica fragrans Houtt. in Allium cepa L.. For mutagenicity test, each juice was dissolved in tap water to make 500, 1000, 2000, and 4000 mgs/kgs (w/v) concentrations while anti-mutagenicity test involved dissolving both the juice and cyclophosphamide (CP) (50 mg/kg) in tap water. Root length and tips of suspended onions in these solutions were measured respectively and prepared for microscopic examination after 48 h of growth. DPPH free radical scavenging activity of the juices was tested using butylated hydroxyanisole with butylated hydroxytoluene as the reference standard. Only M. fragrans’s juice showed better radical scavenging activity close to BHA and BHT at 1 mg/mI. The juices inhibited root growth and cell division in A. cepa. However, there was lesser mite-depressive effect of the combination of the juices, except for A. bilimbi with CP than their ordinary juices on the cell division. Chromosomal aberrations induced were not significantly different from the negative control except at 2000 mg/kg of M. citrifolia and 1000—2000 mg/kg of P acidus. Mutagenic effect of CP was reduced by the juices, but not concentration dependent, except for M. fragrans. The observed mutagenic effects could be due to synergistic reactions between DPPH radical scavengers and other phytochemicals in the juices. However, results of anti-mutagenicity test suggested that the lowering of cytotoxic and mutagenic effects of CP on cell division and chromosome structure of A. cepa by the tested juices was due to antioxidants.

Keywords

Allium cepa; cyclophosphamide; mutagenicity; anti-mutagenicty; antioxidants

Session

Poster Abstracts: P06

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization