Selection Of Cell Lines For Cell Suspension Culture Of Artemisia Annua L. Of Vietnam Origin

Author

Ch’na S.J., Yahaya Z.S., Tran V.M., Boey P.L and Chan L.K.
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia School of Chemical Science, Universiti Sains Malaysia, 11800 Penang, Malaysia
Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City. Vietnam
Tel: 04-6533520 E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Selection Of Cell Lines For Cell Suspension Culture Of Artemisia Annua L. Of Vietnam Origin

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

Artemisia annua L. is the source of artemisinin which is the most efficient anti-malarial drug against the multidrug resistant strains of Plasmodium. Due to the environmental and economical factor, in vitro cell suspension culture technique was reported as one of the alternative ways for mass production of this compound. Selection of the starting material is important for the establishment of cell suspension culture. Cell lines were produced from in vitro leaf explants of three A. annua clones (TC1, TC2 and Hi) which germinated from the seeds collected from two different geographical locations in Vietnam. The growth index of the cell lines were recorded every four weeks by sub-culturing 0.5g of the callus into fresh medium for 13 subculture cycles, The cell lines were categorized into fast, intermediate and slow growing based on the growth index of the callus. The cell lines with consistent growth index were selected for the initiation of cell suspension culture of A. annua.

Keywords

Anti-malarial; artemisinin; cell Lines; callus culture; growth index

Session

Poster Abstracts: P09

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization