Effect of Medium, Light and Subculture Frequency on Callus Growth of Artemisia Annua L.

Author

Chin C.K., Bahrudin S., Boey P.L and Chan L.K.
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang
School of Chemical Sciences,  Universiti Sains Malaysia, 11800 Penang
Tel: 04-6533520 E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Effect of Medium, Light and Subculture Frequency on Callus Growth of Artemisia Annua L.

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

Callus of Artemisia annua has been reported to contain artemisinin, a rare sesquiterpene lactone endoperoxide, currently used in artemisinin-based combination therapies (ACT) against malaria. The trend of callus growth of three varieties of A. annua in two MS-based media was investigated throughout 10 subculture cycle. Growth of Highland variety under darkness and light illumination in four different types of MS-based medium was also studied. Results indicated that different types of supplement in MS medium clearly affected callus growth behaviour. The three varieties showed difference in colour and texture of callus maintained in MS medium added with 0.5 mg/L BA, 0.5 mg/L NAA and 0.5 g/L casein hydrolysate. In MS medium supplemented with 2.0 mg/L picloram. TC1 variety showed distinct changes in callus morphology during the 10 subculture cycle whereas TC2 and Highland varieties did not show any significant change in appearance. Callus growth index of Highland variety in MS medium with 0.5 mg/L BA, 0.5 mg/L NAA and 0.5 g/L casein hydrolysate under light exposure was 11.6, significantly higher than that under darkness with growth index of 8.2. However, callus growth in three other media showed no significant difference between light and dark conditions.

Keywords

Artemisia annua; callus growth; plant growth regulator; casein hydrolysate; picloram

Session

Poster Abstracts: P10

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization