Somatic Embryogenesis of Artemisia Annua L.

Author

Ku Nornadia K.R., Abu Hassan A., Tran V.M., Boey P.L. and Chan L.K.
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
lnstitute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam
Tel: 04-6533520 E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Somatic Embryogenesis of Artemisia Annua L.

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

Artemisia annua L. is one of the most important medicinal plants in the world for its artemisinin, an anti-malarial drug. Commercially available artemisinin is mainly extracted from the wild plants, hence subjected to seasonal and other environmental limitations. Somatic embryogenesis, an in vitro culture technology, provides an alternative to overcome these limitations. Somatic embryogenesis was obtained from leaf callus culture of A. annua. Callus cultures of leaf explants were initiated on induction medium containing Murashige and Skoog (MS) medium supplemented with 0.5 mg/l naphthaleneacetic acid (NAA) and 0.5 mg/I 6-benzylaminopurine (BA), 0.5 g casein hydrolysate and 30 g/l sucrose. Optimum callus production and variations in callus morphology, colour and texture were observed and evaluated. Histological studies confirmed the formation of somatic embryogenesis by callus treated with various concentration of 2,4-dichlorophenoxyacetic acid after one week of culture on basic MS medium without plant growth regulator. Somatic embryos that contained artemisinin will be tested against mosquito larvae.

Keywords

Somatic embryogenesis; Artemisia annua; artemisinin; histology

Session

Poster Abstracts: P13

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization