Optimization of Pressurized Liquid Extraction (PLE) Of Zingiber Zerumbet L.

Author

Norianah Y., Siti Nurlia A. and Khairil A.J.
Department of Applied Chemistry. Faculty of Applied Sciences. Universiti Teknologi MARA, 40450 Shah Alam. Selangor

Language

English

Title

Optimization of Pressurized Liquid Extraction (PLE) Of Zingiber Zerumbet L.

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

Pressurized liquid extraction (PLE) is a technique which involves extraction with solvents at high pressure and temperature without their critical points being reached. Zingiber zerumbet L. is locally known as lempoyang and belongs to the family Zingiberaceae. It is commonly used as Ingredients in Malaysian traditional medicine, health supplements and tonics. The objective of this study was to optimize the extraction of oils from rhizomes of Zingiber zerumbet using PLE under various temperatures (50°C to 80°C) and pressures (1250, 1500, 1750 and 2000 psi). The solvent selected for this study was hexane. The chemical compounds present in extracts of Z. zerumbet were determined by using Gas Chromatography - Mass Spectrometry. The yield of the extracts was analyzed and compared using statistical analysis tool. The optimum pressure and temperature for PLE of Z. zerumbet were 1750 psi and 70°C, respectively. These gave a maximum yield of 14.6%. Sixty chemical compounds were identified from the oils extracted from the rhizomes of Z. zerumbet. The major compounds obtained were zerumbone (60.30% area) at 1750 psi and 80°C. a-humulene (13.10% area) at 1250 psi and 80°C. and ocimene (2.53% area) at 1750 psi and 50°C. The results showed that PLE could be used for simultaneous extraction of zerumbone, α-humulene and ocimene from the rhizomes of Z. zerumbet.

Keywords

Zingiber zerumbet L.; pressurized liquid extraction; optimization

Session

Poster Abstracts: P16

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization