Investigation of Orthosiphon Stamineus for its Chemo-preventive Activity in Human Hepatoma Cell Line Hepg2

Author

Shamsul M, Noor Rain A. and Zakiah I.
Pusat Penyelidikan Perubatan Herba (HMRC), Institutut Penyelidikan Perubatan (IMR), Jalan Pahang, 50588 Kuala Lumpur
Tel: 03-2616 2633/ 014266 2704 E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Investigation of Orthosiphon Stamineus for its Chemo-preventive Activity in Human Hepatoma Cell Line Hepg2

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

In the present study, we investigated the chemo-preventive activity of water extract of Orthosiphon stamineus (WOS) in human hepatoma cell line HepG2. MTS assay indicated that WOS at concentration of 25 to 800 µg/mI showed no effect on the viability of HepG cells in time- and concentration-dependent manner. ELISA assay and Western blot showed that the levels of NFkBp65 nuclear in WOS-treated HepG2 cells were significantly reduced compared to control. The major compound in WOS was found to be rosmarinic acid, which was quantitatively determined by high performance liquid chromatography (HPLC). The content of rosmarinic acid was 18.1% (refered to peak area in chromagram). In conclusion, the results suggested that WOS might have beneficial chemo-preventive properties and worthy of further studies.

Keywords

Orthosiphon stamineus; chemoprevention; HepG2; NFkBp65

Session

Poster Abstracts: P33

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization