Effect of Water Extract of Tinospora Crispa (L.) Hook.F. & Thomson on Two Breast Cancer Cell Lines Mcf-7 and Mda-MB-231

Author

Ibahim M.J., Wan-Nor I’zzah W.M.Z. and Froemming G.A.R.
Institute for Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi
MARA, 40450 Shah Alam, Selangor

Language

English

Title

Effect of Water Extract of Tinospora Crispa (L.) Hook.F. & Thomson on Two Breast Cancer Cell Lines Mcf-7 and Mda-MB-231

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

Medicinal plants have a track record for treating a variety of illnesses and they are excellent starting materials for the identification of new biologically active compounds. Tinospora crispa (L.) Hook.f. & Thomson is a recognized traditional medicinal plant used in India, Indonesia, the Philippines and Malaysia. Yet, there are numerous studies on this plant highlighting its antioxidant, anti-diabetic, anti-malarial and cosmetic effects but inadequate information regarding the cytotoxicity potential of T. crispa. The objective of this study was to determine the anti-proliferative activity of water extracts of T crispa on two breast cancer cell lines. MCF-7 and MDA-MB-231. The normal fibroblast cell line 3T3 serves as control cell. Cell cytotoxicity was determined using MTT assay where tamoxifen was used as positive control. Results obtained showed that the cell viability decreased in a dose dependent manner in each cell line similar to the pattern of tamoxifen. The observed lC50 values for MCF-7, MDA-MB-231 and 3T3 cells were 42.75±4.61, 46.88±1.75 and 65.50±4.64 µg/mI. However, the value obtained for 3T3 was significantly higher compared to MCF-7 and MDA-MB-231 (p< 0.05, df=2, X2 = 8.78). In conclusion, water extract of T. cnspa had a dose dependent anti-proliferative activity against the two breast cancer cell lines, MCF-7 and MDA-MB-231 with the lowest lC50 MCF-7. Further studies are needed to elucidate the mechanism of cell death induced by T.crispa

Keywords

Tinospora crispa; anti-proliferative; MCF-7; MDA-MB-231

Session

Poster Abstracts: P36

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization