Differentiation of Melastoma Malabathricum L. from Different Localities using Ft-Ir and 2d-Ir Correlation Spectroscopy

Author

Salbiah M.,  Ling S.K., Adilah H.Z.A., Tan A.L. & Zunoliza A. Phytochemistly Programme,  Medicinal Plants Division, Forest Research Institute Malaysia,52109 Kepong, Selangor School of Chemical Sciences, University Science Malaysia, 11800 Minden, Pulau Pinang 
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Language

English

Title

Differentiation of Melastoma Malabathricum L. from Different Localities using Ft-Ir and 2d-Ir Correlation Spectroscopy

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

Melastoma malabathricum L. is a shrub that belongs to the family Melastomataceae easily found around tropical regions of the world. It has beautiful pink or white flowers. In this study, we applied the FT-IR and 2D-IR correlation spectroscopy on samples of M. malabthricum collected from Stesen Penyelidikan Luar FRIM Maran (Pahang), Bukit Rotan Kuala Selangor (Selangor), Gunung Ulu Kali Genting Highland (Pahang), Gunung Bnnchang Cameron Highland (Pahang) and Pant Sibor (Johor). The results of the conventional infrared spectroscopy showed limited differences but the second derivative infrared spectra could amplify the differences of M. malabathricum collected from the five different locations. The 1D-IR spectra of M. malabathricum were grouped by using exploratory data analysis (EDA). Furthermore, in the 2D-IR correlation spectroscopic analysis the spectral resolution of 2D-IR spectra under thermal perturbation was greatly enhanced. The comparison of 2D-lR correlation spectra based on the positions and intensities of auto-peaks and cross-peaks as the third step of differentiation revealed more differences among the samples from different locations. The visual colourful 2D-IR spectra can further provide dynamic structural information of chemical components in the samples. Therefore excellent macroscopic fingerprints can be obtained rapidly without going through sample extraction.

Keywords

Melastoma malabathricum; Fourier transform infrared spectroscopy (FT R); Second derivative infrared spectroscopy; Two-dimensional infrared correlation (2D-IR);  Exploratory data analysis (EDA)

Session

Poster Abstracts: P43

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization