Amygladin Content in The Aqueus Extracts of Raw and Dried Apricot Kernel and its Cytotoxic Effect on HT29 Cell

Author

Rohanizah A.R., Faezah A., Ruzita A. and Ishak M. Cancer MAKNA-USM Unit, Advanced Medical & Dental Institute, Universiti Sains Malaysia, 11800 Minden, Penang

Language

English

Title

Amygladin Content in The Aqueus Extracts of Raw and Dried Apricot Kernel and its Cytotoxic Effect on HT29 Cell

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

The kernels of apricot have been shown to contain a number of compounds that have been advocated to possess anti-cancer activities. One of the compounds is a cyanogenic glycoside known as amygdalin, which can also be found in the genus Prunus, such as bitter almond. Amygdalin has been widely used in alternative cancer therapy, although concerns have been raised as to the danger of accidental cyanide poisoning from its use. In folkloric practices, the raw apricot is much more effective than the dried counterpart. In this study, the differences between the raw and dried apricot seed were studied by analyzing the amygdalin content and the cytotoxic effect of its extracts on HT29 cells. High Performance Liquid Chromatography (HPLC) was used to identify and quantify the concentration of amygdalin between two different samples (raw and dry) that are available in the market. The effect of the two different samples at different concentrations (0, 0.625, 1.25, 2.5, 5, 10 and 20 mg/ml) on HT29 cancer cell line were studied using the MTS technique. The samples were tested for 24, 48 and 72 h. Results of HPLC analysis showed that the raw sample had higher concentration of amygdalin compared to the dry sample. The MTS assay results showed that the raw sample inhibited a greater Percentage of cell growth compared to dry sample, at all concentrations tested, for 48 h. The results from the assays also showed that aqueous extracts from the raw and the dry samples as Well as amygdalin itself were not cytotoxic to the HT29 cells, although there were relative minor differences in the level of inhibition of cell growth observed. Therefore, the results from this Study suggested that there were differences in the analyte content and their biological functions etween the raw and the dry apricot.

Keywords

Amygdalin; cytotoxic; MTS technique; HPLC

Session

Poster Abstracts: P46

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization