Comparative Study of Effectiveness Varying Extraction Times and Solvent Ratios of Eurycomanone Extraction from Tongkat Ali

Author

Mohd Radzi A., Nurul Ain A., Ahmad Shukri A. and Abd. Rashid L. Medicinal Plant Division, FRIM, 52109 Kepong, Selangor Management and Science University, Shah Alam, Selangor

Language

English

Title

Comparative Study of Effectiveness Varying Extraction Times and Solvent Ratios of Eurycomanone Extraction from Tongkat Ali

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

Eurycoma Iongifolia Jack or Tongkat Ali is a traditional Malay and indigenous herb used in local traditional medicine against a variety of diseases. This plant is popularly sought for as single ingredient or as an ingredient in mixture with other herbs in herbal remedies. In Malay traditional medicine, the roots of E. longifolia is the main ingredient used to make “jamu” or tonic for health preparation. It is sometimes referred to as the ‘Malaysian Ginseng’. The root of this plant is also used in folk medicine for the treatment of aches, dysentery, glandular swelling, diarrhoea, bleeding, dropsy, persistent coughing, hypertension and as a health supplement. A key issue here is the production of Tongkat Ali extracts that should contain the marker compound that indicates the presence of Tongkat Ali. This standardized extract should have a specific measurable amount of the marker compound and this would help to improve as well as control the quality of Tongkat Ali based products. The chemical marker compound In Tongkat Ali preferred is eurycomanone, a quassinoid which is the primary component present in the extract with a specific measurable amount of bioactivity. The objective of this study is to investigate the yield of eurycomanone extracted from various duration and solvent ratios. The eurycomanone content based on different duration and solvent ratio was analyzed using Ultra Performance Liquid Chromatography (UPLC). The results showed that the optimal ratio was (1:10) and the extraction duration was 3 to 6 h giving sufficient percentage of eurycomanone (4.4 to 4.5%) with RSD of 3.4to 6.3%.

Keywords

Eurycoma longifolia; extraction; Eurycomanone; UPLC

Session

Poster Abstracts: P47

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization