Effect of Different Specimen Preparation Techniques on Yield of Amygdalin Concentration in Apricot Kernel Extract

Author

Syazwani I., Faizah A. and Ishak M. Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia, 11800 USM, Pu/au Pinang Tel: 04-6532724 E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Effect of Different Specimen Preparation Techniques on Yield of Amygdalin Concentration in Apricot Kernel Extract

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

Amygdalin (D-mandelonitrile-β-gentiobioside) is a cyanogenic compound found in majority of seeds of members of the genus Prunus, such as bitter almonds and apricot kernels. Amygdalin is decomposed to mandelonitrile and glucose by β-glucosidase. Semen Armeniacae or the seed of Prunus armeniaca L. not only contains amygdalin but also an emulsin which is an enzyme that hydrolyzes amygdalin. In our study, we quantitively analyzed amygdalin using gas chromatography-mass spectrometry with HP5-MS column. We used four different techniques to prepare the specimens for extraction using 10 gm of apricot kernel for each group. The first group was blended to give fine pieces of the kernel; the second was cut into 12 pieces; the third was cut into 2 half pieces and the fourth was used whole. The four specimens were then incubated in 0.1% citric acid for 3 h in a water bath at 100°C, and the extracts were filtered and subsequently freeze-dried. The extracts were analysed by using Agilent Gas Chromatography- Mass Spectrometry. In general, the extraction efficiency tended to increase as the specimen size decreased. However, in this case, the result was the opposite. The abundance of amygdalin detected from the chromatogram was the highest in group 4 and the least was from group 1. This may be due to the removal of the emulsin from the surface of the shell during extraction. In group 1, the surface emulsin had been mixed together in the fine pieces. The results from this study showed that processing of the raw apricot kernels could be important in maximising the amount of amygdalin that could be extracted from the original source.

Keywords

Apricot kernels; Semen Armeniacae; amygdalin; Gas Chromatograohy-Mass Spectrometry (GCMS)

Session

Poster Abstracts: P51

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization