Anticancer and Antioxidant Properties of Crude Extract of Eugenia aromatica (L.) Baill.

Author

Wan-Nor l’zzah W.M.Z., Froemming G.A.R, Ibahim M.J., Asmah R. and MohdFadzelly A.B. Faculty of Medicine, Universiti Teknologi MARA (U1TM) Level 20, Tower 1, Science & Technology Complex, 40450 Shah A lam, Se/an gor lnstitute of Medical Molecular Biotechnology, U1TM Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah Tel: 03-5544 2836 Fax: 03-5544 2831 E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Language

English

Title

Anticancer and Antioxidant Properties of Crude Extract of Eugenia aromatica (L.) Baill.

Proceeding

Medicinal and Aromatic Plants Seminar (MAPS 2010)

Date

3rd August - 4th August (2010)

Place Held

Forest Research Institute Malaysia(FRIM)

Abstract

Spices are a source of antioxidants. They help to prevent the oxidative stress caused by the reduction process of oxygen that leads to DNA damage and several diseases including cancer. Therefore, they represent a potential source of new compounds with anticancer and antioxidant properties. Methanol crude extract of the flowers of Eugenia aromatica (L.) Baill. or clove was investigated for anticancer and antioxidant activities: The methanol crude extract was tested against four human cancer cell lines, i.e., (HepG2, hepatic cancer; MCF-7, hormone-dependent breast cancer; MDA-MB-231, non-hormone-dependent breast cancer and HeLa, cervical cancer) and the normal cells, 3T3, using the Mfl (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. It was shown that the methanol extract inhibited 50% cancer cell growth at concentration of >100 µg/mI. Of all cancer cell lines tested, methanol extract of E. aromatica was most effective in inhibiting MCF-7. The antioxidant capacity of the methanol extract was investigated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The results showed high scavenging activity against DPPH radical ranging from 90 to 96% at concentrations 10 to 100 µg/mI. The total phenolic content of the methanol extract was determined by the Folin-Ciocalteau method. The result expressed as gallic acid equivalent (GAEs) showed that methanol extract contained 312.73 mg GAEs/g sample. On the other hand, it was shown that the methanol extract was not rich in flavonoids. Results which were expressed as rutin equivalents (REs) showed 21.33 mg REs/g sample. In conclusion, the antioxidant properties of E. aromatica may have a role in its anticancer activities. However, further study is needed to confirm this.

Keywords

Eugenia aromatica: methanol crude extract; anticancer; antioxidant

Session

Poster Abstracts: P52

Topic

Harnessing the Tropical heritage: Recent Advances in R&D and commercialization