The Chelating Effect of Silymarin on Iron Overload in Rats


Ghafghazi T, Najafzaheh H and Moayedi BS.


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia




Silymarin, chelating effect


Iron – catalyzed oxidative stress is believed to be the main mechanism involved in pathogenesis of organ dysfunction in iron overload. The use of antioxidants may prove to be useful in counteracting the oxidative effect of iron and preventing its toxicity. The present study was undertaken to evaluate the protective effect of orally and i.p. administration of silymarin in established model of long term iron overload in rats. HEPG2 cells with RPMI media were used, then Fecl3, silymarin and deferoxamine were added after over night culture. The cells were washed with PBS. After cell lyses, the iron of supernatant solution was measured with spectrophotometric atomic absorption. Six groups of rats were studied for 14 days and were treated with Fecl3 (100 mg / kg i.p.), or Fecl2 plus deferoxamine (50 mg / kg i.p.), silymarin (200 mg / kg oral or i.p.) every other day respectively. The statistical analysis of the iron cell culture shows that silymarin and deferoxamine both decreased the iron concentration of hepatocyte cells. Also it was found that i.p. silymarin was more effective than oral silymarin or i.p. deferoxamin.