Hepatoprotective Activity of Cichorium intybus L. Leaves Extract Against Carbon Tetrachloride Induced Toxicity


Akram Jamshidzadeh, Mohammad Javad Khoshnood and Hossein Niknahad


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia




Cichorium intybus, CCl4, hepatoprotective


The effects of different concentrations of the hydroalcoholic extract of dried powdered leaves ofCichorium intybusL., on CCl4-induced hepatotoxicity in vivo in rats and CCl4-induced cytotoxicity in isolated rat hepatocytes were investigated. Rats received different concentrations of the extract by i.p. injection for 3 consecutive days before the injection of CCl4 3 ml/kg (i.p.). Twenty four hours after CCl4 injection the animals were bled and the livers were dissected for biochemical and histopathological studies. The results showed that theC. intybusextract could protect the liver from CCl4-induced damages with doses of 50 and 100 mg/kg, but concentrations higher than 200 mg/kg were less effective. For in vitro studies, the extract were added to the suspension of freshly isolated rat hepatocytes incubated in Krebs-Henseleit buffer under a ¿ow of 95% O2 and 5% CO2, 20 minutes before the addition of 10 mM of CCl4. The extract with concentrations of 60 to 600 μg/ml protected the cells against CCl4-induced cytotoxicity, but concentrations of 1.5 mg/ml and higher increased the CCl4-induced cytotoxicity. TheC. intybusextract itself was toxic towards isolated hepatocytes with concentrations above 3.6 mg/ml. Therefore, the results of the present study support the traditional believes on hepatoprotective effect of the extract ofC. intybusleaves, however, high concentrations were hepatotoxic.