Potential Insulin-Releasing Property of Psidium guajava Polar Leaf Extracts.


Chee Beng Jin, Abd. Rashid Li, Nor Hayati Abdullah and Rasadah Mat Ali


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia




Psidium guajava, polar extracts, in-vitro studies, insulin-secreting property


Psidium guajava(Myrtaceae) is commonly known as guava or locally called Jambu Batu. Traditionally, it is used for the treatment of diarrhea, dysentery and acute gastrointestinal inflammation. In traditional Chinese medicine,P. guajavaleaves were reportedly used in herbal preparation for diabetes. Recent findings showed that the butanol soluble fraction from the leaves inhibited the increase of plasma glucose level in alloxan diabetic rats and decreased plasma glucose level in glucose tolerance test. This study aims to evaluate the insulin secreting property of polar leaf extracts ofP.guajavain order to understand the possible mechanism behind its hypoglycemic property. The ethanol leaf extract was fractionated into four fractions. They are mainly n-butanol fraction, 2 n-butanol sub-fractions (n-butanol soluble hydrolysable tannin and saponin­riched fractions) and water fraction (water soluble hydrolysable tannin). In the insulin secretory study, the four fractions were tested on insulin-secreting cell-line. The insulin concentrations released from the insulin-secreting cell-line were ranged between 1.731 ± 0.084 μg/l to 1.976 ± 0.1068 μg/l. In conclusion, all the four fractions from theP.guajavaleaves extract possess insulin secreting property beneficial on the treatment and prevention of hyperglycemia in type two diabetes mellitus. More comprehensive pharmacological and chemical studies are required to explore detailed mechanism and active principle responsible for the effect.