An Investigation of The Analgesic and Anti-inflammatory Effects of<em>Heracleum persicum</em>Hydroalcoholic Extract and Essential Oil in Animal Models


Valiollah Hajihashemi, Seyed Ebrahim Sajjadi and Mojtaba Heshmati


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia




Heracleum persicum, analgesic, anti-inflammatory


Heracleum persicumbelongs to umbellifera family. The fruits of this plant contains about 4% essential oil mainly consisting of aliphatic esters such as hexyl butyrate and octyl acetate. The plant also has several furanocoumarines. In this study the hydroalcoholic extract and essential oil of the plant were investigated for their possible anti-inflammatory and analgesicactivities. Male mice (25-35 g) and Wistar rats (150- 200 g) were used. Essential oil and hydroalcoholic extract were prepared by steam distillation and percolation respectively.   The acetic acid-induced writhing response and formalin-induced pawlicking time were used in mice to assess analgesic activity.  For evaluation of anti-inflammatory effect carrageenan-induced rat paw edema was used. Indomethacin and morphine were used as reference drugs. The extract and essential oil were administered i.p. and orally. Essential oil at doses of 50-200 μl/kg and extract at doses of 125-500 mg/kg reduced aceticacid-induced abdominal constrictions in a dose dependent manner. Also the same doses significantly attenuated the painresponse of the second phase of formalin test. Essential oil at doses of 100 and 200 μl/kg and extract at doses of 200 and400 mg/kg significantly (p<0.01) reduced carrageenan-induced paw edema. These results clearly showed that the analgesic and anti-inflammatory effects of the plant extract and essential oil. Since plant extract and essential oil could not relieve the pain of first phase of formalin test, it seems that they lack any opioid compound. The significant effects observed in other tests indicate that the plant extracts are suitable for controlling pain and especially pain of inflammatory origin.