Qualitative and Quantitative Analysis Some Brands of Valerian Pharmaceutical Products


Saeedeh Ghaffari, Somayeh Esmaeili, Farzaneh Naghibi, Mahmoud Mosaddegh


Traditional & Complementary Medicine Exhibition 2007 (TCME 2007), Putra World Trade Centre (PWTC), Kuala Lumpur, Malaysia




HPLC valerenic acid - determination


One of the important active compounds of valerian is valerenic acid which is supposed to be the chemical factor responsiblefor the sedative effect of valerian. The aim of this work is identification and determination of valerenic acid in some brands of valerian pharmaceutical products including tablet, caplet, capsule, drop and comparison them. Following extraction bymaceration method with methanol 80%, thin layer chromatography (TLC) method was used to qualify the valerenic acidcontent of some brands of valerian pharmaceutical products using ethyl acetate- hexane- methanol (0.5: 3: 7) as a mobile phase comparing to the authentic sample. Also high performance liquid chromatography (HPLC) method was used to quantify the valerenic acid content and the operating conditions were C-18 reversed phase (VP- ODS 250L x 4.6mm) column, acetonitrile as mobile phase with constant flow rate of 0.8 ml/min and UV detection at 220 nm. Valerenic acid amount in some brands of German valerian products (Futuran Baldrian tablet, Baldrian Hopfen tablet, Euvegal caplet) was 0.03- 0.16%and in some of  Iranian products (Neurogol tablet, Valerian capsule and Antimigrain Herbal drop) was 0.05- 2.8%. A rapid, simple and utilizable high performance liquid chromatography (HPLC) method was used for determination of valerenic acidin valerian products. All of the brands contain acceptable range of valerenic acid (not less than 0.04%).Calculated daily dose of valerenic acid in solid dosage form in Iranian valerian products was less than German valerian products.